ArticleMolecular biology of the cell2016
Single-particle imaging reveals intraflagellar transport-independent transport and accumulation of EB1 in Chlamydomonas flagella.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 66 citations in OpenAlex.
- Microtubules in Arabidopsis pollen tubes are oriented away from the tube apex and are actin-independent at the cortex.bioRxiv : the preprint server for biology · 2026Article
- Mitotic entry is controlled by the plant-specific phosphatase BSL1 and cyclin-dependent kinase B.Nature plants · 2025Article
- Imaging-based screen identifies novel natural compounds that perturb cell and chloroplast division inMolecular biology of the cell · 2025Article
- Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Cargo adapters expand the transport range of intraflagellar transport.Journal of cell science · 2022Review
- Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex.eLife · 2022Article
- Cilia proteins getting to work - how do they commute from the cytoplasm to the base of cilia?Journal of cell science · 2022Review
- Control of division in Chlamydomonas by cyclin B/CDKB1 and the anaphase-promoting complex.PLoS genetics · 2022Article
- Protein turnover dynamics suggest a diffusion-to-capture mechanism for peri-basal body recruitment and retention of intraflagellar transport proteins.Molecular biology of the cell · 2021Article
- Diffusion rather than intraflagellar transport likely provides most of the tubulin required for axonemal assembly inJournal of cell science · 2020Article
- Cleavage-furrow formation without F-actin inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Establishing and regulating the composition of cilia for signal transduction.Nature reviews. Molecular cell biology · 2019Review
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- Composition, structure and function of the eukaryotic flagellum distal tip.Essays in biochemistry · 2018Review
- In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties.Molecular biology of the cell · 2018Article
- Cilium structure, assembly, and disassembly regulated by the cytoskeleton.The Biochemical journal · 2018Review
- Review
- The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length.Molecular biology of the cell · 2018Article
- The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium.Scientific reports · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The microtubule (MT) plus-end tracking protein EB1 is present at the tips of cilia and flagella; end-binding protein 1 (EB1) remains at the tip during flagellar shortening and in the absence of intraflagellar transport (IFT), the predominant protein transport system in flagella. To investigate how EB1 accumulates at the flagellar tip, we used in vivo imaging of fluorescent protein-tagged EB1 (EB1-FP) in Chlamydomonas reinhardtii. After photobleaching, the EB1 signal at the flagellar tip recovered within minutes, indicating an exchange with unbleached EB1 entering the flagella from the cell body. EB1 moved independent of IFT trains, and EB1-FP recovery did not require the IFT pathway. Single-particle imaging showed that EB1-FP is highly mobile along the flagellar shaft and displays a markedly reduced mobility near the flagellar tip. Individual EB1-FP particles dwelled for several seconds near the flagellar tip, suggesting the presence of stable EB1 binding sites. In simulations, the two distinct phases of EB1 mobility are sufficient to explain its accumulation at the tip. We propose that proteins uniformly distributed throughout the cytoplasm like EB1 accumulate locally by diffusion and capture; IFT, in contrast, might be required to transport proteins against cellular concentration gradients into or out of cilia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.