Evidence map›Paper›PMID 26631479›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015

Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling.

Terri-Leigh Stephen, Nathalie F Higgs, David F Sheehan, Sana Al Awabdh, Guillermo López-Doménech, I Lorena Arancibia-Carcamo, Josef T Kittler

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.

0numbers the graph read from it
0cells of the map it votes in
77citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

77 citing papers in PubMed, 134 citations in OpenAlex.

  1. Context-Dependent modulation of astrocytic CaJournal of computational neuroscience · 2026
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  18. Targeting mitochondrial CaThe Journal of physiology · 2024
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17 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Terri-Leigh StephenDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom.
Nathalie F HiggsDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom.
David F SheehanDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom.
Sana Al AwabdhDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom.
Guillermo López-DoménechDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom.
I Lorena Arancibia-CarcamoDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom.
Josef T KittlerDepartment of Neuroscience, Physiology and Pharmacology, University College London, London, WC1E 6BT, United Kingdom j.kittler@ucl.ac.uk.
University College London · GB

Funding

Biotechnology and Biological Sciences Research CouncilMedical Research Council G0802377Wellcome Trust
6 · The paper itself

Abstract

It is fast emerging that maintaining mitochondrial function is important for regulating astrocyte function, although the specific mechanisms that govern astrocyte mitochondrial trafficking and positioning remain poorly understood. The mitochondrial Rho-GTPase 1 protein (Miro1) regulates mitochondrial trafficking and detachment from the microtubule transport network to control activity-dependent mitochondrial positioning in neurons. However, whether Miro proteins are important for regulating signaling-dependent mitochondrial dynamics in astrocytic processes remains unclear. Using live-cell confocal microscopy of rat organotypic hippocampal slices, we find that enhancing neuronal activity induces transient mitochondrial remodeling in astrocytes, with a concomitant, transient reduction in mitochondrial trafficking, mediated by elevations in intracellular Ca(2+). Stimulating neuronal activity also induced mitochondrial confinement within astrocytic processes in close proximity to synapses. Furthermore, we show that the Ca(2+)-sensing EF-hand domains of Miro1 are important for regulating mitochondrial trafficking in astrocytes and required for activity-driven mitochondrial confinement near synapses. Additionally, activity-dependent mitochondrial positioning by Miro1 reciprocally regulates the levels of intracellular Ca(2+) in astrocytic processes. Thus, the regulation of intracellular Ca(2+) signaling, dependent on Miro1-mediated mitochondrial positioning, could have important consequences for astrocyte Ca(2+) wave propagation, gliotransmission, and ultimately neuronal function.

Indexed as

AnimalsAnimals, NewbornAstrocytesCalcium SignalingCells, CulturedDependovirusEmbryo, MammalianExcitatory Amino Acid AgentsFemaleGlial Fibrillary Acidic ProteinGlutamic AcidHippocampusIntracellular SpaceIn Vitro TechniquesMaleMitochondriaExcitatory Amino Acid AgentsGlial Fibrillary Acidic ProteinGlutamic AcidMitochondrial Proteinsrho GTP-Binding ProteinsRhot1 protein, ratVesicular Glutamate Transport Protein 1astrocytecalciumMiromitochondriamobilityNMDARs

Identifiers

PMID26631479
PMCPMC4666922
OpenAlexW2186763432

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.