ArticleVirology journal2015
Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus.
Article in Virology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Molecular Characterisation of Orf Virus in Goats From Eastern Türkiye.Veterinary medicine and science · 2026Article
- Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry.Pathogens (Basel, Switzerland) · 2025Article
- Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification.Molecules (Basel, Switzerland) · 2024Review
- Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid visual detection ofHeliyon · 2024Article
- Recent advances in diagnostic approaches for orf virus.Applied microbiology and biotechnology · 2023Review
- Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay.Life (Basel, Switzerland) · 2023Article
- Rapid and sensitive detection of pathogenicFish and shellfish immunology reports · 2022Article
- Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics.The Analyst · 2022Article
- Establishment and Application of a Real-Time Recombinase Polymerase Amplification Assay for the Detection of Avian Leukosis Virus Subgroup J.Frontiers in veterinary science · 2022Article
- Tracking SARS-CoV-2: Novel Trends and Diagnostic Strategies.Diagnostics (Basel, Switzerland) · 2021Review
- Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2.Analytica chimica acta · 2021Article
- Development of a reverse-transcription recombinase polymerase amplification assay with a lateral flow assay for rapid detection of avian orthoavulavirus 1.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods.Frontiers in molecular biosciences · 2021Review
- Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections.PLoS neglected tropical diseases · 2020Article
- Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus.BMC veterinary research · 2018Article
- Recombinase polymerase amplification: Basics, applications and recent advances.Trends in analytical chemistry : TRAC · 2018Review
- A recombinase polymerase amplification assay for rapid detection of Crimean-Congo Haemorrhagic fever Virus infection.PLoS neglected tropical diseases · 2017Article
- Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus.Virology journal · 2017Article
- Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus.Virology journal · 2017Article
- Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2.BioMed research international · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOrf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. The rapid detection of ORFV is of great importance in disease control and highly needed. A isothermal molecular diagnostic approach, termed recombinase polymerase amplification (RPA), is considered as an novel and rapid alternative techonology to PCR assay.
resultsIn the present study, a novel fluorescent probe based on RPA assay (ORFV exo RPA assay) was developed. The developed ORFV exo RPA assay was capable of as low as 100 copies of ORFV DNA /reaction and was highly specific, with no cross-reaction with closely related viruses (capripox virus, foot-and-mouth disease virus or peste des petits ruminants virus). Further assessment with clinical samples showed that the developed ORFV exo RPA assay has good correlation with qPCR assays for detection of ORFV.
conclusionsThese results suggest that the developed ORFV exo RPA assay is suitable for rapid detection of ORFV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.