ArticleBMC genetics2015
Genetic diversity, multiplicity of infection and population structure of Schistosoma mansoni isolates from human hosts in Ethiopia.
Article in BMC genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Extensive parasite transmission and variation in a functional receptor associated with drug resistance in endemicScience advances · 2026Article
- Evidence of high genetic diversity among parasite populations in a schistosomiasis hotspot.Microbiology spectrum · 2025Article
- How useful is Nanopore adaptive sampling for sequencingWellcome open research · 2025Article
- MOIRE: a software package for the estimation of allele frequencies and effective multiplicity of infection from polyallelic data.Bioinformatics (Oxford, England) · 2024Article
- Interleukin-13 (IL-13)-A Pleiotropic Cytokine Involved in Wound Healing and Fibrosis.International journal of molecular sciences · 2023Review
- Geographical Influence on Morphometric Variability of Genetically "Pure"Tropical medicine and infectious disease · 2023Article
- Population Genetic Structure and Hybridization ofPathogens (Basel, Switzerland) · 2022Article
- Cryptic population structure and transmission dynamics uncovered for Schistosoma mansoni populations by genetic analyses.Scientific reports · 2022Article
- Population genetic structure of Schistosoma haematobium and Schistosoma haematobium × Schistosoma bovis hybrids among school-aged children in Côte d'Ivoire.Parasite (Paris, France) · 2022Article
- Whole-genome sequencing of Schistosoma mansoni reveals extensive diversity with limited selection despite mass drug administration.Nature communications · 2021Article
- Revisiting density-dependent fecundity in schistosomes using sibship reconstruction.PLoS neglected tropical diseases · 2021Article
- Schistosomiasis Morbidity Hotspots: Roles of the Human Host, the Parasite and Their Interface in the Development of Severe Morbidity.Frontiers in immunology · 2021Review
- Genetic evidence for the role of non-human primates as reservoir hosts for human schistosomiasis.PLoS neglected tropical diseases · 2020Article
- Two-year longitudinal survey reveals high genetic diversity of Schistosoma mansoni with adult worms surviving praziquantel treatment at the start of mass drug administration in Uganda.Parasites & vectors · 2019Article
- Estimating helminth burdens using sibship reconstruction.Parasites & vectors · 2019Article
- Population Structure and Dynamics of Helminthic Infection: Schistosomiasis.Microbiology spectrum · 2019Review
- Population genetic structure of Schistosoma bovis in Cameroon.Parasites & vectors · 2019Article
- Whole genome amplification and exome sequencing of archived schistosome miracidia.Parasitology · 2018Article
- Phenotypic and genotypic monitoring of Schistosoma mansoni in Tanzanian schoolchildren five years into a preventative chemotherapy national control programme.Parasites & vectors · 2017Article
- Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers.PLoS neglected tropical diseases · 2017Article
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7 authors.
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Abstract
backgroundHuman intestinal schistosomiasis caused by Schistosoma mansoni and urinary schistosomiasis caused by Schistosoma haematobium are endemic in Ethiopia. Although schistosomes look morphologically uniform, there is variation in infectivity, egg productivity and virulence due to variation in their genetic make. Knowing the genetic diversity and population structure of S. mansoni isolates will enable to understand and consider the possible variability in terms of infectivity, egg productivity and virulence.
methodsBetween 2010 and 2011, genetic diversity and population structure of Schistosoma mansoni isolates from four endemic areas of Ethiopia was assessed using previously published 11 polymorphic microsatellite loci. Miracidia were hatched from eggs of S. mansoni collected from stools of human subjects residing in Kemissie, Wondo Genet, Ziway and Sille-Elgo villages. DNA was extracted from single miracidium and PCR was run following standard protocol. Allelic polymorphism and population genetic structure was analyzed using different software.
resultAt a population level (i.e. different villages), the mean number of alleles per locus, allelic richness, expected heterozygosity in Hardy-Weinberg equilibrium and pairwise F ST values ranged from 8.5 to 11.5, 3.46-20.8, 0.66-0.73 and 3.57-13.63%, respectively. All analyzes on population genetic structure reveals strong genetic structuration corresponding to the four sampled villages. At infrapopulation level (i.e. different hosts) the mean number of alleles per locus, allelic richness, expected heterozygosity in Hardy-Weinberg equilibrium and F IS values ranged from 3.09 to 7.55, 1-1.96, 0.59-0.73 and 0.1763-0.4989, respectively. Mean estimated genetically unique adult worm pairs within hosts ranged from 66 to 92% revealing the occurrence of infection of a single host with genetically unique multiple S. mansoni strains. The data also indicated the occurrence of genetic variation within inter- and intra-hosts.
conclusionHigh level of genetic diversity and significant population differentiation characterized the S. mansoni isolates of Ethiopia. These results are quite different from previous studies demonstrating that it is difficult to generalize schistosome transmission patterns because epidemiological situation tends to vary. These are important factors to be considered in relation with morbidity, drug resistance or vaccine development.
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