Evidence map›Paper›PMID 26630669›Full record

ArticlePloS one2015

Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia.

Frédéric Poly, Oralak Serichantalergs, Janelle Kuroiwa, Piyarat Pootong, Carl Mason, Patricia Guerry, Craig T Parker

Erratum issuedAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  18. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Frédéric PolyEnteric Diseases Department, Naval Medical Research Center, Silver Spring, MD, United States of America.
Oralak SerichantalergsArmed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Janelle KuroiwaEnteric Diseases Department, Naval Medical Research Center, Silver Spring, MD, United States of America.
Piyarat PootongArmed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Carl MasonArmed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Patricia GuerryEnteric Diseases Department, Naval Medical Research Center, Silver Spring, MD, United States of America.
Craig T ParkerUnited States Department of Agriculture, Agricultural Research Service, Albany, CA, United States of America.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Campylobacter jejuni produces a polysaccharide capsule that is the major determinant of the Penner serotyping scheme. This passive slide agglutination typing system was developed in the early 1980's and was recognized for over two decades as the gold standard for C. jejuni typing. A preliminary multiplex PCR technique covering 17 serotypes was previously developed in order to replace this classic serotyping scheme. Here we report the completion of the multiplex PCR technology that is able to identify all the 47 Penner serotypes types known for C. jejuni. The number of capsule types represented within the 47 serotypes is 35. We have applied this method to a collection of 996 clinical isolates from Thailand, Cambodia and Nepal and were able to successfully determine capsule types of 98% of these.

Indexed as

SerogroupAsia, SoutheasternBacterial CapsulesCampylobacter jejuniMultiplex Polymerase Chain Reaction

Identifiers

PMID26630669
PMCPMC4668122

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.