ArticlePloS one2015
ITS and trnH-psbA as Efficient DNA Barcodes to Identify Threatened Commercial Woody Angiosperms from Southern Brazilian Atlantic Rainforests.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- DNA barcoding and phylogenetic insights into the selected endemic flora of the Western Himalayas.Scientific reports · 2026Article
- 3RAD-Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree GenusEcology and evolution · 2026Article
- Combined genetic and chemical methods boost the precision of tracing illegal timber in Central Africa.Communications earth & environment · 2025Article
- A critical review of conventional and emerging technologies for the detection of contaminants, allergens and adulterants in plant-based milk alternatives.Current research in food science · 2025Review
- Insights from DNA Barcodes-Based Phylogenetic Analysis of Medicinal Plants and Estimation of Their Conservation Status: A Case Study in the Tianshan Wild Forest, China.Plants (Basel, Switzerland) · 2025Article
- Check Your Shopping Cart: DNA Barcoding and Mini-Barcoding for Food Authentication.Foods (Basel, Switzerland) · 2023Article
- Internal Transcribed Spacer (ITS) Region of Nuclear Ribosomal DNA as a Suitable DNA Barcode for Identification of Zanthoxylum armatum DC. from Manipur.Molecular biotechnology · 2022Article
- Molecular Phylogeny, DNA Barcoding, andGenes · 2022Article
- Assessment of ITS2 Region Relevance for Taxa Discrimination and Phylogenetic Inference among Pinaceae.Plants (Basel, Switzerland) · 2022Article
- Challenges in Medicinal and Aromatic Plants DNA Barcoding-Lessons from the Lamiaceae.Plants (Basel, Switzerland) · 2022Review
- Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.BMC plant biology · 2022Article
- Molecular Characterization of Wild and Cultivated Strawberry (Genetics research · 2022Article
- DNA-Based Herbal Teas' Authentication: An ITS2 andPlants (Basel, Switzerland) · 2021Article
- Complete chloroplast genome ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Assessment of the applicability of wood anatomy and DNA barcoding to detect the timber adulterations in Sri Lanka.Scientific reports · 2020Article
- DNA barcode trnH-psbA is a promising candidate for efficient identification of forage legumes and grasses.BMC research notes · 2020Article
- DNA barcoding and TLC as tools to properly identify natural populations of the Mexican medicinal species Galphimia glauca Cav.PloS one · 2019Article
- DNA barcoding of vouchered xylarium wood specimens of nine endangered Dalbergia species.Planta · 2017Article
- DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China.PloS one · 2017Article
- Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes.Frontiers in plant science · 2016Article
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Authors and funding
4 authors.
Funding
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Abstract
The Araucaria Forests in southern Brazil are part of the Atlantic Rainforest, a key hotspot for global biodiversity. This habitat has experienced extensive losses of vegetation cover due to commercial logging and the intense use of wood resources for construction and furniture manufacturing. The absence of precise taxonomic tools for identifying Araucaria Forest tree species motivated us to test the ability of DNA barcoding to distinguish species exploited for wood resources and its suitability for use as an alternative testing technique for the inspection of illegal timber shipments. We tested three cpDNA regions (matK, trnH-psbA, and rbcL) and nrITS according to criteria determined by The Consortium for the Barcode of Life (CBOL). The efficiency of each marker and selected marker combinations were evaluated for 30 commercially valuable woody species in multiple populations, with a special focus on Lauraceae species. Inter- and intraspecific distances, species discrimination rates, and ability to recover species-specific clusters were evaluated. Among the regions and different combinations, ITS was the most efficient for identifying species based on the 'best close match' test; similarly, the trnH-psbA + ITS combination also demonstrated satisfactory results. When combining trnH-psbA + ITS, Maximum Likelihood analysis demonstrated a more resolved topology for internal branches, with 91% of species-specific clusters. DNA barcoding was found to be a practical and rapid method for identifying major threatened woody angiosperms from Araucaria Forests such as Lauraceae species, presenting a high confidence for recognizing members of Ocotea. These molecular tools can assist in screening those botanical families that are most targeted by the timber industry in southern Brazil and detecting certain species protected by Brazilian legislation and could be a useful tool for monitoring wood exploitation.
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