ArticlePloS one2015
Easy and Rapid Detection of Mumps Virus by Live Fluorescent Visualization of Virus-Infected Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- A DirectView focus reduction assay for high-throughput quantification of neutralizing antibodies against influenza A and B viruses.Cell reports methods · 2026Article
- Fluorescence Imaging of Viral Sialidase Activity Using a Fluorogenic Probe.Advances in experimental medicine and biology · 2026Review
- Development of mumps virus-specific sialidase imaging probes through chemical modifications of sialic acid.Scientific reports · 2025Article
- Enzymatic Substrates and Fluorescence Imaging of Influenza Virus Sialidase.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Function of Sialidase Revealed by Sialidase Activity Imaging Probe.International journal of molecular sciences · 2021Review
- Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mumps viruses show diverse cytopathic effects (CPEs) of infected cells and viral plaque formation (no CPE or no plaque formation in some cases) depending on the viral strain, highlighting the difficulty in mumps laboratory studies. In our previous study, a new sialidase substrate, 2-(benzothiazol-2-yl)-4-bromophenyl 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranosidonic acid (BTP3-Neu5Ac), was developed for visualization of sialidase activity. BTP3-Neu5Ac can easily and rapidly perform histochemical fluorescent visualization of influenza viruses and virus-infected cells without an antiviral antibody and cell fixation. In the present study, the potential utility of BTP3-Neu5Ac for rapid detection of mumps virus was demonstrated. BTP3-Neu5Ac could visualize dot-blotted mumps virus, virus-infected cells, and plaques (plaques should be called focuses due to staining of infected cells in this study), even if a CPE was not observed. Furthermore, virus cultivation was possible by direct pick-up from a fluorescent focus. In conventional methods, visible appearance of the CPE and focuses often requires more than 6 days after infection, but the new method with BTP3-Neu5Ac clearly visualized infected cells after 2 days and focuses after 4 days. The BTP3-Neu5Ac assay is a precise, easy, and rapid assay for confirmation and titration of mumps virus.
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Registered trials
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