Evidence map›Paper›PMID 26629549›Full record

ArticleEBioMedicine2015

Human Blastocyst Secreted microRNA Regulate Endometrial Epithelial Cell Adhesion.

Carly Cuman, Michelle Van Sinderen, Michael P Gantier, Kate Rainczuk, Kelli Sorby, Luk Rombauts, Tiki Osianlis, Evdokia Dimitriadis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed, 1 synthesis or guideline pooled it, 133 citations in OpenAlex.

  1. Pooled it
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  7. Noninvasive Biomarkers of Human Embryo Developmental Potential.International journal of molecular sciences · 2025
    Review
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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Carly CumanEmbryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Monash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Molecular and Translational Science, Monash University, Melbourne, Australia.
Michelle Van SinderenEmbryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Department of Molecular and Translational Science, Monash University, Melbourne, Australia.
Michael P GantierDepartment of Molecular and Translational Science, Monash University, Melbourne, Australia ; Centre for Cancer Research, Hudson Institute, Clayton, Victoria 3168 Australia.
Kate RainczukEmbryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia.
Kelli SorbyEmbryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Monash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Anatomy and Developmental Biology, Monash University, Victoria 3800, Australia.
Luk RombautsMonash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Obstetrics and Gynaecology, Monash University, Victoria 3800, Australia.
Tiki OsianlisMonash IVF, Monash Surgical Private Hospital, Clayton, Victoria 3168, Australia ; Department of Obstetrics and Gynaecology, Monash University, Victoria 3800, Australia.
Evdokia DimitriadisEmbryo Implantation Laboratory, Hudson Institute, Clayton, Victoria 3168 Australia ; Department of Anatomy and Developmental Biology, Monash University, Victoria 3800, Australia.
Hudson Institute of Medical Research · AUMonash IVF · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.

Indexed as

Argonaute ProteinsBlastocystCell AdhesionCell Adhesion MoleculesCell LineEmbryo ImplantationEndometriumEpithelial CellsEukaryotic Initiation FactorsFemaleFertilization in VitroHistone DeacetylasesHumansMicroRNAsNectinsRepressor ProteinsAGO1 protein, humanArgonaute ProteinsCell Adhesion MoleculesEukaryotic Initiation FactorsHistone DeacetylasesMicroRNAsMIRN661 microRNA, humanMTA2 protein, humanNECTIN1 protein, humanNectinsRepressor ProteinsEmbryoEndometriumImplantationmicroRNAmiR-661

Identifiers

PMID26629549
PMCPMC4634783
OpenAlexW2157303024

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.