Evidence map›Paper›PMID 26629176›Full record

ArticleInternational journal of clinical and experimental medicine2015

Establishment of lymphatic filarial specific IgG4 indirect ELISA detection method.

Jin Li, Qing-Kuan Wei, Shao-Liang Hu, Ting Xiao, Chao Xu, Xin Liu, Bing-Cheng Huang, Feng-Ju Jia

Abstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jin LiShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Qing-Kuan WeiShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Shao-Liang HuShenZhen Combined Biotech Co, Ltd Shenzhen 518054, China.
Ting XiaoShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Chao XuShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Xin LiuShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Bing-Cheng HuangShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Feng-Ju JiaShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Jiangsu Institute of Parasitic Diseases · CNLifetech Scientific (China) · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo establish the lymphatic filarial specific IgG4 indirect ELISA detection method and develop the kits.

methodsELISA and the developed specific IgG4 reagent was used to explore the best way for detecting filarial specific IgG4. Combined with the production process of commercialized enzyme immunoassay kit to develop economical lymphatic filarial specific IgG4 test kit, and to explore the value of the kit in the laboratory.

resultsWe determined the most optimal detective antigen was Malay adult filarial antigen and the optimal concentration of coating antigen was 1.0 μg/ml. The appropriate serum dilution was 1:20 to 40 and the work titers of specific IgG4 agents was 1:800. We determined the optimal reaction time for substrates and developed a reproducible and stable detection kit with sensitive and specificity, which was easy to operate.

conclusionWe successfully established the lymphatic filarial specific IgG4 indirect ELISA detection method and developed the kits with good reproducibility and stable result, which should be widely applied.

Indexed as

ELISAkitLymphatic filariasisspecific IgG4

Identifiers

PMID26629176
PMCPMC4659064
OpenAlexW2186718389

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.