Evidence map›Paper›PMID 26628994›Full record

ArticleInternational journal of clinical and experimental medicine2015

Lin28B over-expression mediates the repression of let-7 by hepatitis B virus X protein in hepatoma cells.

Gang Wu, Pinbo Huang, Xueming Ju, Zhixi Li, Youyu Wang

Abstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

  1. RNAi Regulator C3PO Promotes Arbovirus Infection in Insect Vectors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Review
  3. Review
  4. LOC101929709 promotes gastric cancer progression by aiding LIN28B to stabilize c-MYC mRNA.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2023
    Article
  5. Review
  6. Review
  7. Article
  8. Cell death pathways and viruses: Role of microRNAs.Molecular therapy. Nucleic acids · 2021
    Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Review
  14. Review
  15. Observational
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Gang WuDepartment of Hepatobiliary Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital 32 Yihuan Road, Chengdu 610072, People's Republic of China.
Pinbo HuangDepartment of Hepatobiliary Surgery, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University 107 Yanjiang West Road, Guangzhou 510120, People's Republic of China.
Xueming JuDepartment of Ultrasound, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital 32 Yihuan Road, Chengdu 610072, People's Republic of China.
Zhixi LiDepartment of Pediatric Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital 32 Yihuan Road, Chengdu 610072, People's Republic of China.
Youyu WangDepartment of Thoracic Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital 32 Yihuan Road, Chengdu 610072, People's Republic of China.
Sun Yat-sen Memorial Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Let-7 microRNA (miRNA) family is frequently downregulated in multiple human tumors, including hepatocellular carcinoma (HCC). Our previous report demonstrated that hepatitis B virus X protein (HBx) suppressed the expression of let-7 in HepG2 hepatoma cells. However, the underlying mechanisms were not elucidated. Lin28B is known to negatively regulate the maturation of let-7, and this prompted us to determine whether HBx acts through Lin28B to suppress let-7. Real-time reverse-transcription polymerase chain reaction (qRT-PCR) was performed to examine let-7 expression before and after treatment with c-Myc-and Lin28B-specific siRNAs in HepG2 cells stably/transiently transfected with HBx. mRNA and protein analyses were employed to determine the correlation of HBx, c-Myc and Lin28B in HCC tissues and cells. Cell cycle and proliferation assays were performed to delineate the consequences of Lin28B repression in HepG2 cells expressing HBx. Lin28B was overexpressed in HBx-transfected cells and HBV-infected liver tissues. HBx-c-Myc-Lin28B axis mediated the repression of let-7 in HepG2 cells. Reduced expression of Lin28B inhibited the growth and cell cycle progression of HepG2 cells by derepressing let-7 and repressing c-Myc. There was not only a preliminary HBx-c-Myc-Lin28B-let-7 pathway but also another possible double-negative feedback loop between c-Myc/Lin28B and let-7 in HepG2 cells transfected with HBx, which together induced the deregulation of let-7. Lin28B has the potential to be a novel molecular target in the treatment of HBV(+) HCC.

Indexed as

HBxhepatocellular carcinomalet-7Lin28B

Identifiers

PMID26628994
PMCPMC4658883
OpenAlexW2341373225

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.