ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2016
Entrapment of alpha1-acid glycoprotein in high-performance affinity columns for drug-protein binding studies.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Studies and analysis of drug-target interactions by affinity chromatography and related techniques: A review.Journal of pharmaceutical analysis · 2026Review
- Chromatographic-Based Binding and Thermodynamic Studies of Antibiotic Micropollutants with Humic Acid Using Affinity Microcolumns.Journal of separation science · 2026Article
- Europium probe binding to serum albumin and αChemical science · 2025Article
- Production of anti-inflammatory films based on cashew gum polysaccharide and polyvinyl alcohol for wound dressing applications.3 Biotech · 2023Article
- Characterization of binding by sulfonylureas with normal or modified human serum albumin using affinity microcolumns prepared by entrapment.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2023Article
- Characterization of drug binding with alphaJournal of chromatography. A · 2021Article
- Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.Advances in chromatography · 2021Article
- Affinity chromatography: A review of trends and developments over the past 50 years.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2020Review
- Development of an on-line immunoextraction/entrapment system for protein capture and use in drug binding studies by high-performance affinity chromatography.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2020Article
- Optimization of protein entrapment in affinity microcolumns using hydrazide-activated silica and glycogen as a capping agent.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2019Article
- Chiral Stationary Phases for Liquid Chromatography: Recent Developments.Molecules (Basel, Switzerland) · 2019Review
- Characterization of solution-phase drug-protein interactions by ultrafast affinity extraction.Methods (San Diego, Calif.) · 2018Article
- Studies of drug interactions with alphaJournal of chromatography. A · 2017Article
- Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications.Clinical chemistry · 2017Review
- Chromatographic studies of drug interactions with alphaJournal of chromatography. A · 2017Article
- Chromatographic Studies of Protein-Based Chiral Separations.Separations · 2016Article
- On-column entrapment of alpha1-acid glycoprotein for studies of drug-protein binding by high-performance affinity chromatography.Analytical and bioanalytical chemistry · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A slurry-based method was developed for the entrapment of alpha1-acid glycoprotein (AGP) for use in high-performance affinity chromatography to study drug interactions with this serum protein. Entrapment was achieved based on the physical containment of AGP in hydrazide-activated porous silica supports and by using mildly oxidized glycogen as a capping agent. The conditions needed for this process were examined and optimized. When this type of AGP column was used in binding studies, the association equilibrium constant (Ka) measured by frontal analysis at pH 7.4 and 37°C for carbamazepine with AGP was found to be 1.0 (±0.5)×10(5)M(-1), which agreed with a previously reported value of 1.0 (±0.1)×10(5)M(-1). Binding studies based on zonal elution were conducted for several other drugs with such columns, giving equilibrium constants that were consistent with literature values. An entrapped AGP column was also used in combination with a column containing entrapped HSA in a screening assay format to compare the binding of various drugs to AGP and HSA. These results also agreed with previous data that have been reported in literature for both of these proteins. The same entrapment method could be extended to other proteins and to the investigation of additional types of drug-protein interactions. Potential applications include the rapid quantitative analysis of biological interactions and the high-throughput screening of drug candidates for their binding to a given protein.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.