Evidence map›Paper›PMID 26627545›Full record

ArticleThe Journal of allergy and clinical immunology2016

Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways.

Darcy R Denner, Naseer Sangwan, Julia B Becker, D Kyle Hogarth, Justin Oldham, Jamee Castillo, Anne I Sperling, Julian Solway, Edward T Naureckas, Jack A Gilbert and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of allergy and clinical immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed, 4 pooled it
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed, 4 syntheses or guidelines pooled it, 158 citations in OpenAlex.

  1. Pooled it
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  17. Microbial influencers: the airway microbiome's role in asthma.The Journal of clinical investigation · 2025
    Review
  18. Review
  19. Article
  20. Article

49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Darcy R DennerSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Naseer SangwanBiosciences Division (BIO), Argonne National Laboratory, Argonne, Ill.
Julia B BeckerSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
D Kyle HogarthSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Justin OldhamSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Jamee CastilloSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Anne I SperlingSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Julian SolwaySection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Edward T NaureckasSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill.
Jack A GilbertBiosciences Division (BIO), Argonne National Laboratory, Argonne, Ill; Departments of Ecology & Evolution and Surgery, University of Chicago, Chicago, Ill; Institute for Genetics, Genomics, and Systems Biology, University of Chicago, Chicago, Ill; Marine Biological Laboratory, Woods Hole, Mass.
Steven R WhiteSection of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Ill. Electronic address: swhite@bsd.uchicago.edu.
University of Chicago · USArgonne National Laboratory · US

Funding

Re-Engineering Translational Research at the University of ChicagoUL1TR000430 · NCATS · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2012 to 2016
$20.2M
RESEARCH TRAINING IN RESPIRATORY BIOLOGYT32HL007605 · NHLBI · UNIVERSITY OF CHICAGO · PI Yun Fang, Gokhan M. Mutlu · 1985 to 2026
$18.1M
Regulation and expression of HLA-G in asthmatic airwaysU19AI095230 · NIAID · UNIVERSITY OF CHICAGO · PI OBER, CAROLE · 2011 to 2016
$10.5M
NCATS NIH HHS UL1 TR000430NHLBI NIH HHS T32 HL007605NIAID NIH HHS U19 AI095230
6 · The paper itself

Abstract

backgroundThe lung has a diverse microbiome that is modest in biomass. This microbiome differs in asthmatic patients compared with control subjects, but the effects of clinical characteristics on the microbial community composition and structure are not clear.

objectivesWe examined whether the composition and structure of the lower airway microbiome correlated with clinical characteristics of chronic persistent asthma, including airflow obstruction, use of corticosteroid medications, and presence of airway eosinophilia.

methodsDNA was extracted from endobronchial brushings and bronchoalveolar lavage fluid collected from 39 asthmatic patients and 19 control subjects, along with negative control samples. 16S rRNA V4 amplicon sequencing was used to compare the relative abundance of bacterial genera with clinical characteristics.

resultsDifferential feature selection analysis revealed significant differences in microbial diversity between brush and lavage samples from asthmatic patients and control subjects. Lactobacillus, Pseudomonas, and Rickettsia species were significantly enriched in samples from asthmatic patients, whereas Prevotella, Streptococcus, and Veillonella species were enriched in brush samples from control subjects. Generalized linear models on brush samples demonstrated oral corticosteroid use as an important factor affecting the relative abundance of the taxa that were significantly enriched in asthmatic patients. In addition, bacterial α-diversity in brush samples from asthmatic patients was correlated with FEV1 and the proportion of lavage eosinophils.

conclusionThe diversity and composition of the bronchial airway microbiome of asthmatic patients is distinct from that of nonasthmatic control subjects and influenced by worsening airflow obstruction and corticosteroid use.

Indexed as

MicrobiotaAdrenal Cortex HormonesAdultAsthmaBacteriaBronchiBronchoalveolar Lavage FluidEosinophiliaFemaleForced Expiratory VolumeHumansMaleRNA, BacterialRNA, Ribosomal, 16SAdrenal Cortex HormonesRNA, BacterialRNA, Ribosomal, 16S16S ribosomal RNAAsthmabacteriacorticosteroidsFEV(1)microbiome

Identifiers

PMID26627545
PMCPMC4860110
OpenAlexW2176112098

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.