ArticleJournal of microbiology (Seoul, Korea)2015
Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII.
Article in Journal of microbiology (Seoul, Korea), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Structural insights into the assembly and function of Retron Ec78 PtuAB.Communications biology · 2026Article
- Identification of candidate nucleomodulins in ESKAPE bacteria -Frontiers in cellular and infection microbiology · 2026Article
- Structural mechanism of the Retron-Eco7 anti-phage defense system.Nature communications · 2025Article
- Phage nuclease-mediated defense activation of the bacterial Retron-Eco7 toxin-antitoxin system.Nucleic acids research · 2025Article
- ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism.Nature communications · 2024Article
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- Selection for Translational Efficiency in Genes Associated with Alphaproteobacterial Gene Transfer Agents.mSystems · 2022Article
- Article
- Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism.Nature microbiology · 2022Article
- Article
- Retron reverse transcriptase termination and phage defense are dependent on host RNase H1.Nucleic acids research · 2022Article
- Transposon-Sequencing Analysis Unveils Novel Genes Involved in the Generation of Persister Cells in Uropathogenic Escherichia coli.Antimicrobial agents and chemotherapy · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exonuclease VII (ExoVII) of Escherichia coli is a single strandspecific DNA nuclease composed of two different subunits: the large subunit, XseA, and the small subunit, XseB. In this study, we found that multicopy single-stranded DNAs (msDNAs), Ec83 and Ec78, are the in vivo substrates of ExoVII; the enzyme cuts the phosphodiester bond between the fourth and fifth nucleotides from the 5'end. We used this msDNA cleavage to assess ExoVII activity in vivo. Both subunits were required for enzyme activity. Expression of XseA without XseB caused cell death, even though no ExoVII activity was detected. The lethality caused by XseA was rescued by surplus XseB. In XseA-induced death, cells were elongated and multinucleated, and their chromosomes were fragmented and condensed; these are the morphological hallmarks of apoptotic cell death in bacteria. A putative caspase recognition sequence (FVAD) was found in XseA, and its hypothetical caspase product with 257 amino acids was as active as the intact protein in inducing cell death. We propose that under ordinary conditions, XseA protects chromosome as a component of the ExoVII enzyme, but in some conditions, the protein causes cell death; the destruction of cell is probably carried out by the amino terminal fragment derived from the cleavage of XseA by caspase-like enzyme.
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