Evidence map›Paper›PMID 26626160›Full record

ArticlePhytochemistry2016

Characterization of an epoxide hydrolase from the Florida red tide dinoflagellate, Karenia brevis.

Pengfei Sun, Cristian Leeson, Xiaoduo Zhi, Fenfei Leng, Richard H Pierce, Michael S Henry, Kathleen S Rein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Phytochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pengfei SunDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA. Electronic address: psun001@fiu.edu.
Cristian LeesonDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
Xiaoduo ZhiDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
Fenfei LengDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA. Electronic address: lengf@fiu.edu.
Richard H PierceMote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA. Electronic address: rich@mote.org.
Michael S HenryMote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA. Electronic address: mhenry@mote.org.
Kathleen S ReinDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA. Electronic address: reink@fiu.edu.

Funding

Pilot--Molecular Monitoring of Toxic DinoflagellatesS11ES011181 · NIEHS · FLORIDA INTERNATIONAL UNIVERSITY · PI REIN, KATHLEEN S · 2001 to 2011
$6.9M
Mechanisms of Transcription-Coupled DNA SupercoilingSC1HD063059 · NICHD · FLORIDA INTERNATIONAL UNIVERSITY · PI LENG, FENFEI · 2009 to 2012
$972k
Transcription-Coupled DNA SupercoilingR15GM109254 · NIGMS · FLORIDA INTERNATIONAL UNIVERSITY · PI LENG, FENFEI · 2014 to 2014
$317k
NICHD NIH HHS 5SC1HD063059-04NICHD NIH HHS SC1 HD063059NIEHS NIH HHS S11 ES011181NIEHS NIH HHS S11 ES11181NIGMS NIH HHS 1R15GM109254-01A1NIGMS NIH HHS R15 GM109254
6 · The paper itself

Abstract

Epoxide hydrolases (EH, EC 3.3.2.3) have been proposed to be key enzymes in the biosynthesis of polyether (PE) ladder compounds such as the brevetoxins which are produced by the dinoflagellate Karenia brevis. These enzymes have the potential to catalyze kinetically disfavored endo-tet cyclization reactions. Data mining of K. brevis transcriptome libraries revealed two classes of epoxide hydrolases: microsomal and leukotriene A4 (LTA4) hydrolases. A microsomal EH was cloned and expressed for characterization. The enzyme is a monomeric protein with molecular weight 44kDa. Kinetic parameters were evaluated using a variety of epoxide substrates to assess substrate selectivity and enantioselectivity, as well as its potential to catalyze the critical endo-tet cyclization of epoxy alcohols. Monitoring of EH activity in high and low toxin producing cultures of K. brevis over a three week period showed consistently higher activity in the high toxin producing culture implicating the involvement of one or more EH in brevetoxin biosynthesis.

Indexed as

DinoflagellidaEpoxide HydrolasesEpoxy CompoundsFloridaHarmful Algal BloomMarine ToxinsMolecular WeightOxocinsPolyether ToxinsbrevetoxinEpoxide HydrolasesEpoxy Compoundsleukotriene A4 hydrolaseMarine ToxinsOxocinsPolyether ToxinsBrevetoxinDinoflagellateDinophyceaeEpoxide hydrolaseKarenia brevisProrocentrum hoffmanianumProtein biochemistry

Identifiers

PMID26626160
PMCPMC4724521

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.