ArticlePloS one2015
Immortalization of Fetal Bovine Colon Epithelial Cells by Expression of Human Cyclin D1, Mutant Cyclin Dependent Kinase 4, and Telomerase Reverse Transcriptase: An In Vitro Model for Bacterial Infection.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Engineering Immortalized Bovine Granulosa Cells Using a Triple-Gene Approach: Mutant CDK4, Cyclin D1, and TERT.Cell biology international · 2026Article
- Establishment of immortalized Egyptian Rousettus bat cell lines.FEBS open bio · 2024Article
- Establishment of bovine 3D enteroid-derived 2D monolayers.Veterinary research · 2022Article
- Establishment methods and research progress of livestock and poultry immortalized cell lines: A review.Frontiers in veterinary science · 2022Review
- Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells.Scientific reports · 2021Article
- Assessing Intestinal Health.Frontiers in veterinary science · 2021Review
- Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor.Frontiers in cell and developmental biology · 2020Article
- Article
- Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon).PloS one · 2019Article
- Human-Derived Corneal Epithelial Cells Expressing Cell Cycle Regulators as a New Resource forFrontiers in genetics · 2019Article
- Article
- Development of in vitro enteroids derived from bovine small intestinal crypts.Veterinary research · 2018Article
- Expression of human mutant cyclin dependent kinase 4, Cyclin D and telomerase extends the life span but does not immortalize fibroblasts derived from loggerhead sea turtle (Caretta caretta).Scientific reports · 2018Article
- Establishment and characterization of cell lines derived from complete hydatidiform mole.International journal of molecular medicine · 2017Article
- A novel method to generate single-cell-derived cancer-associated fibroblast clones.Journal of cancer research and clinical oncology · 2017Article
- Article
- Functional structure and antimicrobial activity of persulcatusin, an antimicrobial peptide from the hard tick Ixodes persulcatus.Parasites & vectors · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cattle are the economically important animals in human society. They are essential for the production of livestock products such as milk and meats. The production efficiency of livestock products is negatively impacted by infection with zoonotic pathogens. To prevent and control infectious diseases, it is important to understand the interaction between cattle tissue and pathogenic bacteria. In this study, we established an in vitro infection model of an immortalized bovine colon-derived epithelial cell line by transducing the cells with lentiviral vectors containing genes encoding cell cycle regulators cyclin D1, mutant cyclin dependent kinase 4 (CDK4), and human telomerase reverse transcriptase (TERT). The established cell line showed continuous cell proliferation, expression of epithelial markers, and an intact karyotype, indicating that the cells maintained their original nature as colon-derived epithelium. Furthermore, we exposed the established cell line to two strains of Salmonella enterica and EHEC. Interestingly, S. Typhimurium showed higher affinity for the established cell line and invaded the cytoplasm than S. Enteritidis. Quantitative RT-PCR revealed that gene expression of Toll-like receptor 1 (TLR1), TLR 2 and TLR 3, whereas TLR 4, 5 and 6 were not detectable in established cells. Our established immortalized colon-derived epithelial cell should be a useful tool for studies evaluating the molecular mechanisms underlying bacterial infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.