ArticleInternational forum of allergy & rhinology2016
Bactericidal antibiotics promote reactive oxygen species formation and inflammation in human sinonasal epithelial cells.
Article in International forum of allergy & rhinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Clarithromycin, trimethoprim, and penicillin and oxidative nucleic acid modifications in humans: randomised, controlled trials.British journal of clinical pharmacology · 2017Trial
- Role of Reactive Oxygen Species in Chronic Rhinosinusitis: A Narrative Review.Current issues in molecular biology · 2026Review
- Differential Regulation of Oxidative Burst by First Line Drugs Used Against Multi Drug-Resistant Tuberculosis in Naïve Human Innate Immune Cells.Antibiotics (Basel, Switzerland) · 2026Article
- Regulation of Necroptosis by Aspergillus fumigatus Exopolysaccharide in Human Macrophages Derived from THP-1 Cells: Involvement of the RIP1-RIP3-MLKL Signaling Axis.Current microbiology · 2025Article
- Causal relationships between allergic and autoimmune diseases with chronic rhinosinusitis.Scientific reports · 2024Article
- A novel ruthenium-silver based antimicrobial potentiates aminoglycoside activity againstmSphere · 2023Article
- Effects ofViruses · 2022Article
- Azithromycin and Ceftriaxone Differentially Activate NLRP3 in LPS Primed Cancer Cells.International journal of molecular sciences · 2022Article
- Sorafenib inhibits LPS-induced inflammation by regulating Lyn-MAPK-NF-kB/AP-1 pathway and TLR4 expression.Cell death discovery · 2022Article
- Article
- Understanding the Reciprocal Interplay Between Antibiotics and Host Immune System: How Can We Improve the Anti-Mycobacterial Activity of Current Drugs to Better Control Tuberculosis?Frontiers in immunology · 2021Review
- Antibiotics Affect ROS Production and Fibroblast Migration in anFrontiers in cellular and infection microbiology · 2020Article
- Mitochondrial Metabolism in the Intestinal Stem Cell Niche-Sensing and Signaling in Health and Disease.Frontiers in cell and developmental biology · 2020Review
- Is greater antibiotic therapy prior to ESS associated with differences in surgical outcomes in CRS?The Laryngoscope · 2019Observational
- Intact Cell Lipidomics Reveal Changes to the Ratio of Cardiolipins to Phosphatidylinositols in Response to Kanamycin in HeLa and Primary Cells.Chemical research in toxicology · 2018Article
- Impact of antibiotics on smell dysfunction.World journal of otorhinolaryngology - head and neck surgery · 2018Article
- Bactericidal antibiotics promote oxidative damage and programmed cell death in sinonasal epithelial cells.International forum of allergy & rhinology · 2017Article
- Examination of high-antibiotic users in a multi-institutional cohort of chronic rhinosinusitis patients.International forum of allergy & rhinology · 2017Observational
- Reversal of cigarette smoke extract-induced sinonasal epithelial cell barrier dysfunction through Nrf2 Activation.International forum of allergy & rhinology · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBactericidal antibiotics have been shown to stimulate reactive oxygen species (ROS) formation in mammalian cells through mitochondrial dysfunction. This results in oxidative tissue damage that may have negative consequences for long-term antibiotic use. Antibiotics are widely and heavily used in the treatment of acute and chronic sinusitis; however, the relationship between antibiotics and ROS formation in sinonasal epithelial cells (SNECs) has not yet been demonstrated.
methodsHuman SNECs were collected from patients during endoscopic sinus surgery and grown in culture at the air-liquid interface. Differentiated SNECs were stimulated with the bactericidal antibiotics amoxicillin and levofloxacin and the bacteriostatic antibiotic clarithromycin for 24 hours. ROS were quantified via fluorescence. Cell death was quantified by lactate dehydrogenase (LDH) secretion. Expression of inflammatory markers such as tumor necrosis factor α (TNF-α) and nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant genes were measured by real-time polymerase chain reaction (RT-PCR).
resultsCultured SNECs treated with the bactericidal antibiotics amoxicillin and levofloxacin resulted in a significant increase in production of ROS (p < 0.05) and secretion of LDH (p < 0.05). The increase in ROS formation correlated with an increase in expression of Nrf2-mediated antioxidant genes as well as the expression and production of proinflammatory cytokine TNF-α, and interleukin 1 β (IL-1β) (p < 0.05). SNECs treated with clarithromycin did not demonstrate statistically significant increases in ROS or proinflammatory cytokine production.
conclusionIn this study, we show that treatment of cultured human SNECs with bactericidal antibiotics leads to formation of ROS with an associated increase in inflammatory and antioxidant gene expression and cell death. This suggests that long-term or inappropriate antibiotic use in the treatment of sinusitis may result in oxidative tissue damage to the sinonasal epithelium. Future studies will explore the clinical implications of such damage to the sinonasal epithelium.
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