ArticlePLoS neglected tropical diseases2015
Molecular Diagnosis of Pathogenic Sporothrix Species.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers, 2 of them syntheses that pooled it.
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Who cites it
110 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- The Historical Burden of Sporotrichosis in Brazil: a Systematic Review of Cases Reported from 1907 to 2020.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Pooled it
- Sporotrichosis in Mexico.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Pooled it
- Susceptibility Profiles Among Sporothrix brasiliensis Genotypes in the Ongoing Zoonotic Sporotrichosis Epidemic in Brazil, Argentina and Paraguay.Mycopathologia · 2026Article
- Molecular and Epidemiological Surveillance of Sporothrix spp. in Cats and Dogs from Southern Brazil.Mycopathologia · 2026Article
- Osteoarticular Sporotrichosis in a Brazilian Endemic Setting: Associated Factors and Outcomes.Mycoses · 2026Article
- Molecular detection of medically relevant Sporothrix species in roadkilled wildlife in the Brazilian Atlantic forest.Mycopathologia · 2026Article
- Unusual Sporotrichosis: A New Concept Proposal on the Unexpected Faces ofJournal of fungi (Basel, Switzerland) · 2026Review
- Emergence of difficult-to-treat lymphocutaneous sporotrichosis? Analysis based on three cases with high and low MIC Sporothrix brasiliensis isolates.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Temporal tendencies and spatial patterns of human sporotrichosis in Rio de Janeiro State, Brazil, 2007 to 2023.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Diabetes impairs antifungal immunity and drives fatal disseminated sporotrichosis in mice.Frontiers in immunology · 2026Article
- Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- In vitro effects of the new antifungal compound manogepix (APX001) against the three most clinically relevant species of Sporothrix.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Detection of Sporothrix brasiliensis in Human Tissue by Direct PCR Using Species-Specific Primers and NESTED PCR in a New Epidemic Area of Sporotrichosis.Mycopathologia · 2025Article
- Clinical and Molecular Characterization of Feline Sporotrichosis in the Brazilian Amazon: PCR-Based Identification ofAnimals : an open access journal from MDPI · 2025Article
- A novel approach for feline sporotrichosis pathogen detection based on loop-mediated isothermal amplification.Veterinary dermatology · 2025Article
- Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand.PLoS neglected tropical diseases · 2025Article
- Extracutaneous sporotrichosis.Clinical microbiology reviews · 2025Review
- Allochthonous zoonotic sporotrichosis in the Brazilian Central-West: diagnostic challenges in a nonendemic area.Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Article
- Genetic diversity and molecular evolution of 3-carboxymuconate cyclase (Gp60-70), the major antigen in pathogenicMycology · 2025Article
- Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSporotrichosis is a chronic (sub)cutaneous infection caused by thermodimorphic fungi in the order, Ophiostomatales. These fungi are characterized by major differences in routes of transmission, host predilections, species virulence, and susceptibilities to antifungals. Sporothrix species emerge in the form of outbreaks. Large zoonoses and sapronoses are ongoing in Brazil and China, respectively. Current diagnostic methods based on morphology and physiology are inaccurate due to closely related phenotypes with overlapping components between pathogenic and non-pathogenic Sporothrix. There is a critical need for new diagnostic tools that are specific, sensitive, and cost-effective. METHODOLOGY: We developed a panel of novel markers, based on calmodulin (CAL) gene sequences, for the large-scale diagnosis and epidemiology of clinically relevant members of the Sporothrix genus, and its relative, Ophiostoma. We identified specific PCR-based markers for S. brasiliensis, S. schenckii, S. globosa, S. mexicana, S. pallida, and O. stenoceras. We employed a murine model of disseminated sporotrichosis to optimize a PCR assay for detecting Sporothrix in clinical specimens.
resultsPrimer-BLAST searches revealed candidate sequences that were conserved within a single species. Species-specific primers showed no significant homology with human, mouse, or microorganisms outside the Sporothrix genus. The detection limit was 10-100 fg of DNA in a single round of PCR for identifying S. brasiliensis, S. schenckii, S. globosa, S. mexicana, and S. pallida. A simple, direct PCR assay, with conidia as a source of DNA, was effective for rapid, low-cost genotyping. Samples from a murine model of disseminated sporotrichosis confirmed the feasibility of detecting S. brasiliensis and S. schenckii DNA in spleen, liver, lungs, heart, brain, kidney, tail, and feces of infected animals.
conclusionsThis PCR-based method could successfully detect and identify a single species in samples from cultures and from clinical specimens. The method proved to be simple, high throughput, sensitive, and accurate for diagnosing sporotrichosis.
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