Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan.

A E El Tigani-Asil, S M El Sanousi, M A Aljameel, H El Beir, A Adam, M M Abdallatif, M E Hamid

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In one paragraph

Article in Open veterinary journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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5 · Who and what money

Authors and funding

7 authors.

A E El Tigani-AsilDepartments of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia.

S M El SanousiDepartments of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Sudan.

M A AljameelDepartment of Pathology, Veterinary Research laboratory - Nyala, National Veterinary Research Corporation, Sudan.

H El BeirDepartment of Tuberculosis, Institute of Tropical Diseases, Ministry of Science and Technology, Sudan.

A AdamDepartment of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan.

M M AbdallatifDepartment of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan.

M E HamidDepartment of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.

Indexed as

Acid fast bacteriaBovine farcyNontuberculous mycobacteriaPyogenic infection

Identifiers

PMID26623334

PMCPMC4629588

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.