ArticlePeerJ2015
Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Discovering novel reproductive genes in a non-model fly usingFrontiers in genetics · 2022Article
- Evaluating the lettuce metatranscriptome with MinION sequencing for future spaceflight food production applications.NPJ microgravity · 2021Article
- A Comprehensive Multi-Omic Approach Reveals a Relatively Simple Venom in a Diet Generalist, the Northern Short-Tailed Shrew, Blarina brevicauda.Genome biology and evolution · 2020Article
- Cell Separations and Sorting.Analytical chemistry · 2020Review
- Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies.Scientific data · 2019Article
- De novo clustering of long reads by gene from transcriptomics data.Nucleic acids research · 2019Article
- From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy.Genome biology · 2018Review
- IDP-denovo: de novo transcriptome assembly and isoform annotation by hybrid sequencing.Bioinformatics (Oxford, England) · 2018Article
- Real-time DNA barcoding in a rainforest using nanopore sequencing: opportunities for rapid biodiversity assessments and local capacity building.GigaScience · 2018Article
- Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells.Nature communications · 2017Article
- poRe GUIs for parallel and real-time processing of MinION sequence data.Bioinformatics (Oxford, England) · 2017Article
- Article
- Investigation of chimeric reads using the MinION.F1000Research · 2017Article
- The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community.Genome biology · 2016Article
- Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing.Scientific reports · 2016Article
- Advantages of Array-Based Technologies for Pre-Emptive Pharmacogenomics Testing.Microarrays (Basel, Switzerland) · 2016Review
- MinION Analysis and Reference Consortium: Phase 1 data release and analysis.F1000Research · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Portable DNA sequencers such as the Oxford Nanopore MinION device have the potential to be truly disruptive technologies, facilitating new approaches and analyses and, in some cases, taking sequencing out of the lab and into the field. However, the capabilities of these technologies are still being revealed. Here we show that single-molecule cDNA sequencing using the MinION accurately characterises venom toxin-encoding genes in the painted saw-scaled viper, Echis coloratus. We find the raw sequencing error rate to be around 12%, improved to 0-2% with hybrid error correction and 3% with de novo error correction. Our corrected data provides full coding sequences and 5' and 3' UTRs for 29 of 33 candidate venom toxins detected, far superior to Illumina data (13/40 complete) and Sanger-based ESTs (15/29). We suggest that, should the current pace of improvement continue, the MinION will become the default approach for cDNA sequencing in a variety of species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.