Evidence map›Paper›PMID 26622432›Full record

ArticleExperimental and therapeutic medicine2015

Effect of shRNA-mediated knockdown of vascular endothelial growth factor on the proliferation of choroid-retinal endothelial cells under hypoxic conditions.

Ai-Hua Liu, Jing Sun, Y I Shi, Guo-Ling Sun, Hong Zhang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ai-Hua LiuDepartment of Glaucoma and Fundus Diseases, Tianjin Medical University Eye Hospital, Tianjin 300384, P.R. China.
Jing SunDepartment of Glaucoma and Fundus Diseases, Tianjin Medical University Eye Hospital, Tianjin 300384, P.R. China.
Y I ShiDepartment of Glaucoma and Fundus Diseases, Tianjin Medical University Eye Hospital, Tianjin 300384, P.R. China.
Guo-Ling SunDepartment of Glaucoma and Fundus Diseases, Tianjin Medical University Eye Hospital, Tianjin 300384, P.R. China.
Hong ZhangDepartment of Glaucoma and Fundus Diseases, Tianjin Medical University Eye Hospital, Tianjin 300384, P.R. China.
Tianjin Medical University · CNTianjin Medical University Eye Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to investigate the role of vascular endothelial growth factor (VEGF) in cell proliferation under hypoxic conditions. Gene knockdown of VEGF was conducted in the choroid-retinal endothelial RF/6A cell line by transfection with short hairpin RNA (shRNA), in which a shRNA fragment against VEGF was synthesized and cloned into the vector, pSilencer 2.1-U6 neo. Subsequently, a model of hypoxia was established in the RF/6A cell line via treatment with CoCl

Indexed as

cell proliferationCoCl2retinal neovascularization diseasesshort hairpin RNAvascular endothelial growth factor

Identifiers

PMID26622432
PMCPMC4533154
OpenAlexW1920475174

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.