Evidence map›Paper›PMID 26622301›Full record

ArticleIranian journal of parasitology

Recombinant LPG3 Stimulates IFN-Γ and TNF-Α Secretion by Human NK Cells.

Sanaz Rasolzadeh, Mostafa Haji Fatahaliha, Maryam Hosseini, Reza Jafari, Abolfazl Miahipour, Sevil Sadreddini, Zohreh Babalo, Hossein Samadi Kafil, Mehdi Yousefi

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Sanaz RasolzadehDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Mostafa Haji FatahalihaDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Maryam HosseiniDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Reza JafariDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Abolfazl MiahipourDept. of Medical Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Sevil SadreddiniDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Zohreh BabaloImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Hossein Samadi KafilDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Mehdi YousefiImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Tabriz University of Medical Sciences · IRJahrom University of Medical Sciences · IRPasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNatural killer (NK) cells play an important role in early stages of innate immune responses against viral and tumoral attacks. Activation of NK cells by leishmaniasis results in secretion of cytokines such as interferon (IFN)-γ and tumor necrosis factor (TNF)-α, which enhance the phagocytosis and clearance of parasite. Lipophosphoglycan 3 (LPG3), the Leishmania homologous with GRP94 (glucose regulated protein 94), a member of HSP90 family, contributes to LPG assembly as the most abundant macromolecule on the surface of Leishmania promastigotes.

methodsWe purified NK cells from healthy individuals (n=10) using magnetic-activated cell sorting (MACS) technology. Purified NK cells were co-incubated with different concentrations of recombinant LPG3 (rLPG3), and its N-terminal (NT) and C-terminal (CT) fragments. Finally, the production of IFN-γ and TNF-α by NK cells were measured by ELISA.

resultsRecombinant LPG3 but not its fragments (CT and NT), could significantly enhance the production of TNF-α by NK cells (P<0.05). Moreover, rLPG3, CT, and NT fragments were markedly stimulated the secretion of IFN-γ by NK cells (P<0.001).

conclusionThe Leishmania LPG3 antigen could effectively activate NK cells, in vitro. Leishmania LPG3 participates in the innate immunity against leishmaniasis and thereby improves the effective parasite destruction. However, its efficiency should be tested in vivo.

Indexed as

LeishmaniaLPG3Natural killer cellTLR

Identifiers

PMID26622301
PMCPMC4662746
OpenAlexW2197992967

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.