ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2016
Genotyping-by-sequencing map permits identification of clubroot resistance QTLs and revision of the reference genome assembly in cabbage (Brassica oleracea L.).
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 90 citations in OpenAlex.
- Genetic and Epigenetic Mechanisms Underpinning Biotic Stress Resilience ofPlants (Basel, Switzerland) · 2025Review
- Identification and characterization ofHorticulture research · 2025Article
- Clubroot resistant in cruciferous crops: recent advances in genes and QTLs identification and utilization.Horticulture research · 2025Article
- Fine mapping and candidate gene analysis of the major QTL qSW-A03 for seed weight in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Genetic Relationships and Molecular Signatures of Divergence in Traditional Landraces and Morphotypes ofPlants (Basel, Switzerland) · 2024Article
- Map-based cloning and functional analysis of a major quantitative trait locus, BolC.Pb9.1, controlling clubroot resistance in a wild Brassica relative (Brassica macrocarpa).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Environmental conditions modulate the effect of epigenetic factors controlling the response ofFrontiers in plant science · 2024Article
- Identification of QTLs for resistance to 10 pathotypes of Plasmodiophora brassicae in Brassica oleracea cultivar ECD11 through genotyping-by-sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
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- Multi-Omics Approaches to Improve Clubroot Resistance in Brassica with a Special Focus onInternational journal of molecular sciences · 2022Review
- Genotyping by sequencing-based linkage map construction and identification of quantitative trait loci for yield-related traits and oil content in Jatropha (Jatropha curcas L.).Molecular biology reports · 2022Article
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- Development of Ogura CMS restorers in Brassica oleracea subspecies via direct RfoTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops.International journal of molecular sciences · 2021Review
- Identification of Candidate Genes for Clubroot-Resistance inFrontiers in plant science · 2021Article
- Identification of Two Major QTLs inFrontiers in plant science · 2021Article
- Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance inFrontiers in plant science · 2021Article
- Genome-Wide Analysis of Simple Sequence Repeats in Cabbage (Frontiers in plant science · 2021Article
- A high-quality reference genome for cabbage obtained with SMRT reveals novel genomic features and evolutionary characteristics.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clubroot is a devastating disease caused by Plasmodiophora brassicae and results in severe losses of yield and quality in Brassica crops. Many clubroot resistance genes and markers are available in Brassica rapa but less is known in Brassica oleracea. Here, we applied the genotyping-by-sequencing (GBS) technique to construct a high-resolution genetic map and identify clubroot resistance (CR) genes. A total of 43,821 SNPs were identified using GBS data for two parental lines, one resistant and one susceptible lines to clubroot, and 18,187 of them showed >5× coverage in the GBS data. Among those, 4,103 were credibly genotyped for all 78 F2 individual plants. These markers were clustered into nine linkage groups spanning 879.9 cM with an average interval of 1.15 cM. Quantitative trait loci (QTLs) survey based on three rounds of clubroot resistance tests using F2 : 3 progenies revealed two and single major QTLs for Race 2 and Race 9 of P. brassicae, respectively. The QTLs show similar locations to the previously reported CR loci for Race 4 in B. oleracea but are in different positions from any of the CR loci found in B. rapa. We utilized two reference genome sequences in this study. The high-resolution genetic map developed herein allowed us to reposition 37 and 2 misanchored scaffolds in the 02-12 and TO1000DH genome sequences, respectively. Our data also support additional positioning of two unanchored 3.3 Mb scaffolds into the 02-12 genome sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.