ArticleProceedings of the National Academy of Sciences of the United States of America1989
"Bundle blot" purification and initial protein characterization of hair cell stereocilia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 87 citations in OpenAlex.
- Electron cryo-tomography of vestibular hair-cell stereocilia.Journal of structural biology · 2019Article
- Integrative subcellular proteomic analysis allows accurate prediction of human disease-causing genes.Genome research · 2016Article
- Assembly of hair bundles, an amazing problem for cell biology.Molecular biology of the cell · 2015Review
- Gene Expression by Mouse Inner Ear Hair Cells during Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Article
- Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice.Human molecular genetics · 2015Article
- Chaperone-enhanced purification of unconventional myosin 15, a molecular motor specialized for stereocilia protein trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia.Nature · 2012Article
- An adenylyl cyclase signaling pathway predicts direct dopaminergic input to vestibular hair cells.Neuroscience · 2010Article
- Muscarinic acetylcholine receptor subtype expression in avian vestibular hair cells, nerve terminals and ganglion cells.Neuroscience · 2007Article
- Article
- The micromachinery of mechanotransduction in hair cells.Annual review of neuroscience · 2007Review
- The significance of the calcium signal in the outer hair cells and its possible role in tinnitus of cochlear origin.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2004Review
- Lighting up the senses: FM1-43 loading of sensory cells through nonselective ion channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Myosin-1c interacts with hair-cell receptors through its calmodulin-binding IQ domains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Putting ion channels to work: mechanoelectrical transduction, adaptation, and amplification by hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Two mechanisms for transducer adaptation in vertebrate hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The endogenous calcium buffer and the time course of transducer adaptation in auditory hair cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Endogenous buffers limit the spread of free calcium in hair cells.Biophysical journal · 1997Article
- Unconventional myosins in inner-ear sensory epithelia.The Journal of cell biology · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Stereocilia were isolated from bullfrog (Rana catesbeiana) saccular hair cells by nitrocellulose adhesion. The high purity and high yield of the preparation were demonstrated by microscopy. SDS/PAGE of stereociliary proteins resolved 12-15 major bands. Actin, previously identified as a component of the stereociliary core, was identified in purified stereocilia as a band comigrating with authentic actin and by phalloidin labeling of intact isolated stereocilia. Fimbrin was identified in immunoblots of purified stereocilia. The most abundant other proteins migrated at 11, 14, 16-19, 27, and 36 kDa. Demembranated stereociliary cores consisted primarily of protein bands corresponding to actin and fimbrin and several proteins ranging from 43 to 63 kDa. Because the adaptation mechanism in hair cells is calcium-sensitive and seems localized to stereocilia, we sought evidence for calcium-binding proteins in stereocilia. Calmodulin and calbindin antibodies labeled stereocilia in intact cells. A protein band in purified stereocilia exhibited a Ca2+-dependent shift in electrophoretic mobility identical to that of authentic calmodulin, and the 27-kDa band may represent calbindin. These biochemical data demonstrate that stereocilia consist of a relatively small set of proteins. Most of these, including those involved in transduction and adaptation, are as yet uncharacterized. The availability of purified stereocilia should prove useful in further studies of structure-function relationships in these mechanically sensitive organelles.
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