ArticleProceedings of the National Academy of Sciences of the United States of America1989
Expression of the chicken vimentin gene in transgenic mice: efficient assembly of the avian protein into the cytoskeleton.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation.Stem cell research & therapy · 2019Article
- Cellular transcripts of chicken brain tissues in response to H5N1 and Newcastle disease virus infection.Virology journal · 2012Article
- Computational prediction of splicing regulatory elements shared by Tetrapoda organisms.BMC genomics · 2009Article
- Insights into the beaded filament of the eye lens.Experimental cell research · 2007Review
- The function of intermediate filaments in cell shape and cytoskeletal integrity.The Journal of cell biology · 1996Article
- Inhibition of desmin expression blocks myoblast fusion and interferes with the myogenic regulators MyoD and myogenin.The Journal of cell biology · 1994Article
- Assembly of amino-terminally deleted desmin in vimentin-free cells.The Journal of cell biology · 1990Article
- Overexpression of the vimentin gene in transgenic mice inhibits normal lens cell differentiation.The Journal of cell biology · 1989Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To study expression and function of the vimentin gene, transgenic mice were generated by microinjecting the entire chicken gene plus 2.4 kilobases of 5' and 2.6 kilobases of 3' flanking sequences. All the transgenic mice obtained had incorporated multiple copies of the gene. RNA analyses demonstrated that the chicken vimentin gene was efficiently expressed in an appropriate tissue-specific pattern and that the transcripts were properly processed, as in chicken, giving rise to two RNAs. The vimentin transgene was predominantly expressed in lens at levels of up to 10-fold the endogenous level in every transgenic line studied. The chicken vimentin transcripts were efficiently translated into polypeptides that were modified posttranslationally and could assemble into the mouse cytoskeleton. Overexpression of the chicken vimentin gene did not obviously affect the expression of the endogenous gene at the RNA or the protein level. Immunofluorescence microscopy further demonstrated that the chicken protein was properly expressed spatially in lens. However, the levels were much higher in the transgenic animals.
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Registered trials
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