ArticleProceedings of the National Academy of Sciences of the United States of America2015
Production of functional small interfering RNAs by an amino-terminal deletion mutant of human Dicer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 59 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.
- Pooled it
- Tuning intracellular immunity by Nodamura virus B2 protein enhances self-amplifying RNA activity.Nature communications · 2026Article
- Induction of antiviral RNA interference by avian coronavirus IBV in PBMCs-Mφ.Cell communication and signaling : CCS · 2026Article
- Host-virus molecular arms race: RNAi-mediated antiviral defense and viral suppressor of RNAi.Cell insight · 2025Review
- Dicer is cleaved by the Leader protease encoded by foot-and-mouth disease virus to promote infection in mammalian cells.Science advances · 2025Article
- Virus-derived siRNA: Coronavirus and influenza virus trigger antiviral RNAi immunity in birds.Nucleic acids research · 2025Article
- Enhanced RNAi does not provide efficient innate antiviral immunity in mice.Nucleic acids research · 2025Article
- Catch me if you can: viral nucleic acids to host sensors.Frontiers in immunology · 2025Review
- The human Dicer helicase domain is capable of ATP hydrolysis and single-stranded nucleic acid binding.BMC biology · 2024Article
- The helicase domain of human Dicer prevents RNAi-independent activation of antiviral and inflammatory pathways.The EMBO journal · 2024Article
- microRNAs in action: biogenesis, function and regulation.Nature reviews. Genetics · 2023Review
- Interplay between RNA interference and transposable elements in mammals.Frontiers in immunology · 2023Review
- Structural and functional basis of mammalian microRNA biogenesis by Dicer.Molecular cell · 2022Article
- STUB1 regulates antiviral RNAi through inducing ubiquitination and degradation of Dicer and AGO2 in mammals.Virologica Sinica · 2022Article
- Mouse circulating extracellular vesicles contain virus-derived siRNAs active in antiviral immunity.The EMBO journal · 2022Article
- Untangling the roles of RNA helicases in antiviral innate immunity.PLoS pathogens · 2021Review
- Upping the ante on mammalian antiviral RNA interference.Cell host & microbe · 2021Article
- Efficient Dicer processing of virus-derived double-stranded RNAs and its modulation by RIG-I-like receptor LGP2.PLoS pathogens · 2021Article
- An isoform of Dicer protects mammalian stem cells against multiple RNA viruses.Science (New York, N.Y.) · 2021Article
- Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Although RNA interference (RNAi) functions as a potent antiviral innate-immune response in plants and invertebrates, mammalian somatic cells appear incapable of mounting an RNAi response and few, if any, small interfering RNAs (siRNAs) can be detected. To examine why siRNA production is inefficient, we have generated double-knockout human cells lacking both Dicer and protein kinase RNA-activated. Using these cells, which tolerate double-stranded RNA expression, we show that a mutant form of human Dicer lacking the amino-terminal helicase domain can process double-stranded RNAs to produce high levels of siRNAs that are readily detectable by Northern blot, are loaded into RNA-induced silencing complexes, and can effectively and specifically inhibit the expression of cognate mRNAs. Remarkably, overexpression of this mutant Dicer, but not wild-type Dicer, also resulted in a partial inhibition of Influenza A virus-but not poliovirus-replication in human cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.