ReviewAdvances in experimental medicine and biology2015
Mapping the Protein-Protein Interactome Networks Using Yeast Two-Hybrid Screens.
Review in Advances in experimental medicine and biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Insights into the human cDNA: A descriptive study using library screening in yeast.Journal, genetic engineering & biotechnology · 2024Article
- The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus.Nature communications · 2023Article
- Screening and characterisation of proteins interacting with the mitogen-activated protein kinase Crmapk in the fungus Clonostachys chloroleuca.Scientific reports · 2022Article
- A comprehensive SARS-CoV-2-human protein-protein interactome network identifies pathobiology and host-targeting therapies for COVID-19.Research square · 2022Article
- Two Liberibacter Proteins Combine to Suppress Critical Innate Immune Defenses in Citrus.Frontiers in plant science · 2022Article
- Article
- Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens.Journal of visualized experiments : JoVE · 2018Article
- A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions.Journal of visualized experiments : JoVE · 2018Article
- Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella.Parasites & vectors · 2018Article
- Mechanisms of action of Coxiella burnetii effectors inferred from host-pathogen protein interactions.PloS one · 2017Article
- DEEPN as an Approach for Batch Processing of Yeast 2-Hybrid Interactions.Cell reports · 2016Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
The yeast two-hybrid system (Y2H) is a powerful method to identify binary protein-protein interactions in vivo. Here we describe Y2H screening strategies that use defined libraries of open reading frames (ORFs) and cDNA libraries. The array-based Y2H system is well suited for interactome studies of small genomes with an existing ORFeome clones preferentially in a recombination based cloning system. For large genomes, pooled library screening followed by Y2H pairwise retests may be more efficient in terms of time and resources, but multiple sampling is necessary to ensure comprehensive screening. While the Y2H false positives can be efficiently reduced by using built-in controls, retesting, and evaluation of background activation; implementing the multiple variants of the Y2H vector systems is essential to reduce the false negatives and ensure comprehensive coverage of an interactome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.