ArticleScientific reports2015
In vitro and in vivo screening for novel essential cell-envelope proteins in Pseudomonas aeruginosa.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- ThemBio · 2026Article
- Activation of the envelope stress-responsive two-component system AmgRS compensates for depletion of the essential lipoprotein signal peptidase LspA inCurrent research in microbial sciences · 2026Article
- Retargeting Gram-Positive-Only Adarotene-Derived Antibacterials to Broad-Spectrum Antibiotics.Antibiotics (Basel, Switzerland) · 2025Article
- The Genetic Background and Culture Medium Only Marginally Affect the In Vitro Evolution ofAntibiotics (Basel, Switzerland) · 2025Article
- LolA and LolB are conserved in Bacteroidota and are crucial for gliding motility and Type IX secretion.Communications biology · 2025Article
- Redundant essentiality of AsmA-like proteins inmSphere · 2024Article
- Unrealized targets in the discovery of antibiotics for Gram-negative bacterial infections.Nature reviews. Drug discovery · 2023Review
- Differential Virulence of Aggregatibacter actinomycetemcomitans Serotypes Explained by Exoproteome Heterogeneity.Microbiology spectrum · 2023Article
- Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Inactivation of the Mla system and outer-membrane phospholipase A results in disrupted outer-membrane lipid asymmetry and hypervesiculation inCurrent research in microbial sciences · 2022Article
- Facilitating Compound Entry as a Means to Discover Antibiotics for Gram-Negative Bacteria.Accounts of chemical research · 2021Review
- Identification of Drug Resistance Determinants in a Clinical Isolate of Pseudomonas aeruginosa by High-Density Transposon Mutagenesis.Antimicrobial agents and chemotherapy · 2020Article
- Genetic Basis and Physiological Effects of Lipid A Hydroxylation inPathogens (Basel, Switzerland) · 2019Article
- Functional characterization of a putative DNA methyltransferase, EadM, in Xanthomonas axonopodis pv. glycines by proteomic and phenotypic analyses.Scientific reports · 2019Article
- Functional characterization and proteomic analysis of lolA in Xanthomonas campestris pv. campestris.BMC microbiology · 2019Article
- Pseudomonas aeruginosa LptE is crucial for LptD assembly, cell envelope integrity, antibiotic resistance and virulence.Virulence · 2018Article
- Ferric Uptake Regulator Fur Is Conditionally Essential in Pseudomonas aeruginosa.Journal of bacteriology · 2017Article
- Using bacterial genomes and essential genes for the development of new antibiotics.Biochemical pharmacology · 2017Review
- Bioinformatics and Drug Discovery.Current topics in medicinal chemistry · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-negative bacterium Pseudomonas aeruginosa represents a prototype of multi-drug resistant opportunistic pathogens for which novel therapeutic options are urgently required. In order to identify new candidates as potential drug targets, we combined large-scale transposon mutagenesis data analysis and bioinformatics predictions to retrieve a set of putative essential genes which are conserved in P. aeruginosa and predicted to encode cell envelope or secreted proteins. By generating unmarked deletion or conditional mutants, we confirmed the in vitro essentiality of two periplasmic proteins, LptH and LolA, responsible for lipopolysaccharide and lipoproteins transport to the outer membrane respectively, and confirmed that they are important for cell envelope stability. LptH was also found to be essential for P. aeruginosa ability to cause infection in different animal models. Conversely, LolA-depleted cells appeared only partially impaired in pathogenicity, indicating that this protein likely plays a less relevant role during bacterial infection. Finally, we ruled out any involvement of the other six proteins under investigation in P. aeruginosa growth, cell envelope stability and virulence. Besides proposing LptH as a very promising drug target in P. aeruginosa, this study confirms the importance of in vitro and in vivo validation of potential essential genes identified through random transposon mutagenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.