ArticleThe Journal of cell biology2015
An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- The microcephaly protein Abnormal Spindle has an essential role in symmetrically dividing neural precursors to promote brain growth and development.Developmental biology · 2026Article
- Orb2 RNA-binding activity promotes neural stem cell development and brain growth in Drosophila larvae.Cell reports · 2026Article
- Microcephaly-associated genes asp and Sas4 influence chromatin organization and nuclear lamina structure in Drosophila melanogaster.Development (Cambridge, England) · 2026Article
- A MOPD II-associated Pericentrin variant disrupts PACT domain dimerization and pericentriolar material recruitment.bioRxiv : the preprint server for biology · 2026Article
- Review
- The PP2A-B56 Binding Site LxxIxE Contributes to Asp-Mediated Spindle Pole Stability.Cytoskeleton (Hoboken, N.J.) · 2025Article
- A deep learning model for accurate segmentation of the Drosophila melanogaster brain from Micro-CT imaging.Developmental biology · 2025Article
- The CPEB ortholog Orb2 regulates brain size through the TRIM-NHL RNA-binding protein, Brain tumor.bioRxiv : the preprint server for biology · 2025Article
- Mutations in abnormal spindle disrupt temporal transcription factor expression and trigger immune responses in the Drosophila brain.Genetics · 2023Article
- Review
- The neurological and non-neurological roles of the primary microcephaly-associated protein ASPM.Frontiers in neuroscience · 2023Review
- Pericentrin interacts with Kinesin-1 to drive centriole motility.The Journal of cell biology · 2022Article
- Article
- Understanding microcephaly through the study of centrosome regulation in Drosophila neural stem cells.Biochemical Society transactions · 2020Review
- UsingDevelopment (Cambridge, England) · 2020Review
- Micro-computed tomography as a platform for exploringDevelopment (Cambridge, England) · 2019Article
- Fascetto interacting protein ensures proper cytokinesis and ploidy.Molecular biology of the cell · 2019Article
- Emerging mechanisms of asymmetric stem cell division.The Journal of cell biology · 2018Review
- Same but different: pleiotropy in centrosome-related microcephaly.Molecular biology of the cell · 2018Review
- Nasser Rusan: Scoping out centrosomes.The Journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The interaction between centrosomes and mitotic spindle poles is important for efficient spindle formation, orientation, and cell polarity. However, our understanding of the dynamics of this relationship and implications for tissue homeostasis remains poorly understood. Here we report that Drosophila melanogaster calmodulin (CaM) regulates the ability of the microcephaly-associated protein, abnormal spindle (Asp), to cross-link spindle microtubules. Both proteins colocalize on spindles and move toward spindle poles, suggesting that they form a complex. Our binding and structure-function analysis support this hypothesis. Disruption of the Asp-CaM interaction alone leads to unfocused spindle poles and centrosome detachment. This behavior leads to randomly inherited centrosomes after neuroblast division. We further show that spindle polarity is maintained in neuroblasts despite centrosome detachment, with the poles remaining stably associated with the cell cortex. Finally, we provide evidence that CaM is required for Asp's spindle function; however, it is completely dispensable for Asp's role in microcephaly suppression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.