ArticlePloS one2015
Protocol Improvements for Low Concentration DNA-Based Bioaerosol Sampling and Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
- Surface-Engineered Filters for Wettability-Driven Collection of Airborne Fungal Spores.Global challenges (Hoboken, NJ) · 2026Article
- Deep longitudinal lower respiratory tract microbiome profiling reveals genome-resolved functional and evolutionary dynamics in critical illness.Nature communications · 2024Article
- Bioaerosol Sampling Devices and Pretreatment for Bacterial Characterization: Theoretical Differences and a Field Experience in a Wastewater Treatment Plant.Microorganisms · 2024Article
- An overview for monitoring and prediction of pathogenic microorganisms in the atmosphere.Fundamental research · 2024Review
- Microbial antibiotic resistance genes across an anthropogenic gradient in a Canadian High Arctic watershed.Sustainable microbiology · 2024Article
- Expanding a Wastewater-Based Surveillance Methodology for DNA Isolation from a Workflow Optimized for SARS-CoV-2 RNA Quantification.Journal of biomolecular techniques : JBT · 2023Article
- An Optimized Active Sampling Procedure for Aerobiological DNA Studies.Sensors (Basel, Switzerland) · 2023Article
- AirDNA sampler: An efficient and simple device enabling high-yield, high-quality airborne environment DNA for metagenomic applications.PloS one · 2023Article
- Article
- Experimental parameters defining ultra-low biomass bioaerosol analysis.NPJ biofilms and microbiomes · 2021Article
- Exposure to traffic-related air pollution and bacterial diversity in the lower respiratory tract of children.PloS one · 2021Article
- Article
- Taxonomic composition and seasonal dynamics of the air microbiome in West Siberia.Scientific reports · 2020Article
- Metagenomics Reveals a Core Macrolide Resistome Related to Microbiota in Chronic Respiratory Disease.American journal of respiratory and critical care medicine · 2020Article
- Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens.BMC microbiology · 2020Article
- Performance evaluation of a new custom, multi-component DNA isolation method optimized for use in shotgun metagenomic sequencing-based aerosol microbiome research.Environmental microbiome · 2020Article
- Microbial communities in the tropical air ecosystem follow a precise diel cycle.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Bioaerosol biomonitoring: Sampling optimization for molecular microbial ecology.Molecular ecology resources · 2019Article
- Methods to Investigate the Global Atmospheric Microbiome.Frontiers in microbiology · 2019Article
- Exploring temporal patterns of bacterial and fungal DNA accumulation on a ventilation system filter for a Singapore university library.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionAs bioaerosol research attracts increasing attention, there is a need for additional efforts that focus on method development to deal with different environmental samples. Bioaerosol environmental samples typically have very low biomass concentrations in the air, which often leaves researchers with limited options in choosing the downstream analysis steps, especially when culture-independent methods are intended.
objectivesThis study investigates the impacts of three important factors that can influence the performance of culture-independent DNA-based analysis in dealing with bioaerosol environmental samples engaged in this study. The factors are: 1) enhanced high temperature sonication during DNA extraction; 2) effect of sampling duration on DNA recoverability; and 3) an alternative method for concentrating composite samples. In this study, DNA extracted from samples was analysed using the Qubit fluorometer (for direct total DNA measurement) and quantitative polymerase chain reaction (qPCR). RESULTS AND
findingsThe findings suggest that additional lysis from high temperature sonication is crucial: DNA yields from both high and low biomass samples increased up to 600% when the protocol included 30-min sonication at 65°C. Long air sampling duration on a filter media was shown to have a negative impact on DNA recoverability with up to 98% of DNA lost over a 20-h sampling period. Pooling DNA from separate samples during extraction was proven to be feasible with margins of error below 30%.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.