ArticleNature methods2016
Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.
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Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73citing papers in PubMed
4.8field-weighted citation impact, top 4% of its field
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73 citing papers in PubMed, 124 citations in OpenAlex.
- Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography.IUCrJ · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Review
- Present and future structural biology activities at DESY and the European XFEL.Journal of synchrotron radiation · 2025Article
- Cyclic Olefin Copolymer-Based Fixed-Target Sample Delivery Device for Protein X-ray Crystallography.Analytical chemistry · 2024Article
- Changes in an enzyme ensemble during catalysis observed by high-resolution XFEL crystallography.Science advances · 2024Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Structural insights into functional properties of the oxidized form of cytochrome c oxidase.Nature communications · 2023Article
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography.bioRxiv : the preprint server for biology · 2023Article
- Room temperature X-ray absorption spectroscopy of metalloenzymes with drop-on-demand sample delivery at XFELs.Pure and applied chemistry. Chimie pure et appliquee · 2023Article
- Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.Lab on a chip · 2023Article
- Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source.Scientific reports · 2023Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Capturing the sequence of events during the water oxidation reaction in photosynthesis using XFELs.FEBS letters · 2023Review
13 more citing papers are in PubMed but not listed here.
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Authors and funding
30 authors at 5 institutions in 2 countries.
Raymond G SierraStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Cornelius GatiCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Hamburg, Germany.
Hartawan LaksmonoStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
E Han DaoStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Sheraz GulLawrence Berkeley National Laboratory, Berkeley, California, USA.
Franklin FullerLawrence Berkeley National Laboratory, Berkeley, California, USA.
Jan KernLawrence Berkeley National Laboratory, Berkeley, California, USA.
Ruchira ChatterjeeLawrence Berkeley National Laboratory, Berkeley, California, USA.
Mohamed IbrahimInstitute für Biologie, Humboldt University of Berlin, Berlin, Germany.
Aaron S BrewsterLawrence Berkeley National Laboratory, Berkeley, California, USA.
Iris D YoungLawrence Berkeley National Laboratory, Berkeley, California, USA.
Tara Michels-ClarkLawrence Berkeley National Laboratory, Berkeley, California, USA.
Andrew AquilaLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Mengning LiangLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Mark S HunterLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Jason E KoglinLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0001-6811-6083
Sébastien BoutetLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Elia A JuncoLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Brandon HayesLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Michael J BoganStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Christina Y HamptonStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Elisabetta V PuglisiDepartment of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
Nicholas K SauterLawrence Berkeley National Laboratory, Berkeley, California, USA.
Claudiu A StanStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Athina ZouniInstitute für Biologie, Humboldt University of Berlin, Berlin, Germany.
Junko YanoLawrence Berkeley National Laboratory, Berkeley, California, USA.
Vittal K YachandraLawrence Berkeley National Laboratory, Berkeley, California, USA.
S Michael SoltisStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Joseph D PuglisiDepartment of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
Hasan DeMirciStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0002-9135-5397
SLAC National Accelerator Laboratory · USLawrence Berkeley National Laboratory · USHumboldt-Universität zu Berlin · DEStanford University · USUniversität Hamburg · DE
Funding
X-ray Cryst. CoreP50GM082545 · NIGMS · UNIVERSITY OF UTAH · PI SUNDQUIST, WESLEY I. · 2007 to 2018
$51.1MZWITTERION SURFACTANT DDMAB FOR IMPROV STABIL OF PROTEINP41RR001209 · NCRR · STANFORD UNIVERSITY · PI GONZALEZ, ANA M · 1985 to 2011
$38.9MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MTaking Snapshots of the Water-Oxidation Reaction by the Mn4Ca Oxygen-Evolving Complex Using X-ray Crystallography and SpectroscopyR01GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 1997 to 2022
$9.1MSTRUCTURAL BASIS FOR RIBOSOME FUNCTIONSR01GM051266 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI PUGLISI, JOSEPH D · 1995 to 2021
$7.7MBiological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8MRealizing New Horizons in X-ray Crystallography Data ProcessingR01GM095887 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2011 to 2014
$1.5MTowards real-time XFEL data reduction with CCTBXR01GM102520 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2012 to 2015
$1.4MStructure and Function of the Mn Oxygen-Evolving ComplexR56GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 2009 to 2009
$530kNCRR NIH HHS P41RR001209NIGMS NIH HHS GM055302NIGMS NIH HHS GM082545NIGMS NIH HHS GM095887NIGMS NIH HHS GM102520NIGMS NIH HHS GM110501NIGMS NIH HHS GM51266NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41GM103393NIGMS NIH HHS P50 GM082545NIGMS NIH HHS R01 GM051266NIGMS NIH HHS R01 GM055302NIGMS NIH HHS R01 GM095887NIGMS NIH HHS R01 GM102520NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R56 GM055302
Abstract
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).
Indexed as
CrystallographyModels, MolecularPhotosystem II Protein ComplexRibosomesPhotosystem II Protein Complex
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