ArticleJournal of the American Chemical Society2015
Single Enzyme Experiments Reveal a Long-Lifetime Proton Leak State in a Heme-Copper Oxidase.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Assessing the mechanism of facilitated proton transport across GUVs trapped in a microfluidic device.Biophysical journal · 2024Article
- Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors.Bio-protocol · 2023Article
- Rapid and Highly Stable Membrane Reconstitution by LAiR Enables the Study of Physiological Integral Membrane Protein Functions.ACS central science · 2023Article
- Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy.ACS applied materials & interfaces · 2022Article
- Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs.Macromolecules · 2022Article
- Single-particle combinatorial multiplexed liposome fusion mediated by DNA.Nature chemistry · 2022Article
- Impact of Hydrogen Sulfide on Mitochondrial and Bacterial Bioenergetics.International journal of molecular sciences · 2021Review
- Biased cytochrome P450-mediated metabolism via small-molecule ligands binding P450 oxidoreductase.Nature communications · 2021Article
- Electrophysiology Measurements of Metal Transport by MntH2 fromMembranes · 2020Article
- Lipid Composition Affects the Efficiency in the Functional Reconstitution of the CytochromeInternational journal of molecular sciences · 2020Article
- Constructing artificial respiratory chain in polymer compartments: Insights into the interplay betweenProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A large size-selective DNA nanopore with sensing applications.Nature communications · 2019Article
- Extremely High Frequency Electromagnetic Fields Facilitate Electrical Signal Propagation by Increasing Transmembrane Potassium Efflux in an Artificial Axon Model.Scientific reports · 2018Article
- Spherical-supported membranes as platforms for screening against membrane protein targets.Analytical biochemistry · 2018Article
- Direct observation of multiple conformational states in Cytochrome P450 oxidoreductase and their modulation by membrane environment and ionic strength.Scientific reports · 2018Article
- Direct observation of proton pumping by a eukaryotic P-type ATPase.Science (New York, N.Y.) · 2016Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Heme-copper oxidases (HCOs) are key enzymes in prokaryotes and eukaryotes for energy production during aerobic respiration. They catalyze the reduction of the terminal electron acceptor, oxygen, and utilize the Gibbs free energy to transport protons across a membrane to generate a proton (ΔpH) and electrochemical gradient termed proton motive force (PMF), which provides the driving force for the adenosine triphosphate (ATP) synthesis. Excessive PMF is known to limit the turnover of HCOs, but the molecular mechanism of this regulatory feedback remains relatively unexplored. Here we present a single-enzyme study that reveals that cytochrome bo3 from Escherichia coli, an HCO closely homologous to Complex IV in human mitochondria, can enter a rare, long-lifetime leak state during which proton flow is reversed. The probability of entering the leak state is increased at higher ΔpH. By rapidly dissipating the PMF, we propose that this leak state may enable cytochrome bo3, and possibly other HCOs, to maintain a suitable ΔpH under extreme redox conditions.
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Registered trials
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