ArticleInternational journal of clinical and experimental pathology2015
Proteomic analysis of synovial fluid as an analytical tool to detect candidate biomarkers for knee osteoarthritis.
Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Synovial Fluid Biomarkers in Knee Osteoarthritis: A Systematic Review and Quantitative Evaluation Using BIPEDs Criteria.Cartilage · 2021Pooled it
- Synovial Fluid Proteomic Biomarkers in Knee Osteoarthritis: A Systematic Review and Gene Ontology Analysis.Cartilage · 2026Review
- Molecular Dynamics Modeling of Potential Osteoarthritic Biomarkers.Life (Basel, Switzerland) · 2025Article
- Exploring the Early Molecular Pathogenesis of Osteoarthritis Using Differential Network Analysis of Human Synovial Fluid.Molecular & cellular proteomics : MCP · 2024Article
- Serum proteomic biomarkers diagnostic of knee osteoarthritis.Osteoarthritis and cartilage · 2024Article
- Comparative transcriptome analysis provides insight into the molecular targets and signaling pathways of deer TGF-1 regulating chondrocytes proliferation and differentiation.Molecular biology reports · 2023Article
- Introducing Matrix Metalloproteinases as Crucial Targets for Intra-articular Laser Therapy in Patients with Synovial Fluid of Knee Osteoarthritis.Medical journal of the Islamic Republic of Iran · 2023Article
- Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits.PeerJ · 2022Article
- Identification of Potential Therapeutic Target Genes in Osteoarthritis.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Fetal Immunomodulatory Environment Following Cartilage Injury-The Key to CARTILAGE Regeneration?International journal of molecular sciences · 2021Article
- Comparison of bone and articular cartilage changes in osteoarthritis: a micro-computed tomography and histological study of surgically and chemically induced osteoarthritic rabbit models.Journal of orthopaedic surgery and research · 2021Article
- Nfib promotes chondrocyte proliferation and inhibits differentiation by mildly regulating Sox9 and its downstream genes.Molecular biology reports · 2021Article
- Identifying the hub gene and immune infiltration of osteoarthritis by bioinformatical methods.Clinical rheumatology · 2021Article
- Poor Clearance of Free Hemoglobin Due to Lower Active Haptoglobin Availability is Associated with Osteoarthritis Inflammation.Journal of inflammation research · 2021Article
- Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis.Life (Basel, Switzerland) · 2020Article
- Metabolomic profiles of induced pluripotent stem cells derived from patients with rheumatoid arthritis and osteoarthritis.Stem cell research & therapy · 2019Article
- Review
- Article
- Application of global metabolomic profiling of synovial fluid for osteoarthritis biomarkers.Biochemical and biophysical research communications · 2018Article
- Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation.Arthritis research & therapy · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We conducted research to detect the proteomic profiles in synovial fluid (SF) from knee osteoarthritis (OA) patients to better understand the pathogenesis and aetiology of OA. Our long-term goal is to identify reliable candidate biomarkers for OA in SF. The SF proteins obtained from 10 knee OA patients and 10 non-OA patients (9 of whom were patients with a meniscus injury in the knee; 1 had a discoid meniscus in the knee, and all exhibited intact articular cartilage) were separated by two-dimensional electrophoresis (2-DE). The repeatability of the obtained protein spots regarding their intensity was tested via triplicate 2-DE of selected samples. The observed protein expression patterns were subjected to statistical analysis, and differentially expressed protein spots were identified via matrix-assisted laser desorption/ionisation-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS). Our analyses showed low intrasample variability and clear intersample variation. Among the protein spots observed on the gels, there were 29 significant differences, of which 22 corresponded to upregulation and 7 to downregulation in the OA group. One of the upregulated protein spots was confirmed to be haptoglobin by mass spectrometry, and the levels of haptoglobin in SF are positively correlated with the severity of OA (r = 0.89, P < 0.001). This study showed that 2-DE could be used under standard conditions to screen SF samples and identify a small subset of proteins in SF that are potential markers associated with OA. Spots of interest identified by mass spectrometry, such as haptoglobin, may be associated with OA severity.
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Identifiers
26617706PMC4637791W2425678594What OpenQuestion holds
Registered trials
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