ArticleEnvironmental microbiology2016
Functional metagenomic selection of ribulose 1, 5-bisphosphate carboxylase/oxygenase from uncultivated bacteria.
Article in Environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Deep-branching evolutionary intermediates reveal structural origins of form I rubisco.Current biology : CB · 2023Article
- Design and Construction of Artificial Biological Systems for One-Carbon Utilization.Biodesign research · 2023Review
- Structural plasticity enables evolution and innovation of RuBisCO assemblies.Science advances · 2022Article
- Approaches to Unmask Functioning of the Uncultured Microbial Majority From Extreme Habitats on the Seafloor.Frontiers in microbiology · 2022Review
- Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for COBioresources and bioprocessing · 2021Article
- Metagenomic analysis revealed the sulfur- and iron- oxidation capabilities of heterotrophic denitrifying sludge.Ecotoxicology (London, England) · 2021Article
- Engineering of a chromogenic enzyme screening system based on an auxiliary indole-3-carboxylic acid monooxygenase.MicrobiologyOpen · 2019Article
- Selection of Cyanobacterial (mBio · 2019Article
- Thawing out frozen metabolic accidents.BMC biology · 2019Review
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
- Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacteriumThe Journal of biological chemistry · 2017Article
- The Diverse AAA+ Machines that Repair Inhibited Rubisco Active Sites.Frontiers in molecular biosciences · 2017Review
- Unraveling RubisCO Form I and Form II Regulation in an Uncultured Organism from a Deep-Sea Hydrothermal Vent via Metagenomic and Mutagenesis Studies.Frontiers in microbiology · 2017Article
- Harnessing the power of microbial autotrophy.Nature reviews. Microbiology · 2016Review
- Biochemical and synthetic biology approaches to improve photosynthetic COCurrent opinion in chemical biology · 2016Review
- RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha.The FEBS journal · 2016Article
Corrections and comments
- Commented on by
Authors and funding
12 authors at 7 institutions in 2 countries.
Funding
Abstract
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) is a critical yet severely inefficient enzyme that catalyses the fixation of virtually all of the carbon found on Earth. Here, we report a functional metagenomic selection that recovers physiologically active RubisCO molecules directly from uncultivated and largely unknown members of natural microbial communities. Selection is based on CO2 -dependent growth in a host strain capable of expressing environmental deoxyribonucleic acid (DNA), precluding the need for pure cultures or screening of recombinant clones for enzymatic activity. Seventeen functional RubisCO-encoded sequences were selected using DNA extracted from soil and river autotrophic enrichments, a photosynthetic biofilm and a subsurface groundwater aquifer. Notably, three related form II RubisCOs were recovered which share high sequence similarity with metagenomic scaffolds from uncultivated members of the Gallionellaceae family. One of the Gallionellaceae RubisCOs was purified and shown to possess CO2 /O2 specificity typical of form II enzymes. X-ray crystallography determined that this enzyme is a hexamer, only the second form II multimer ever solved and the first RubisCO structure obtained from an uncultivated bacterium. Functional metagenomic selection leverages natural biological diversity and billions of years of evolution inherent in environmental communities, providing a new window into the discovery of CO2 -fixing enzymes not previously characterized.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.