ArticleJournal of molecular biology2016
Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex.
Article in Journal of molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A new Epseptimavirus bacteriophage vB_SalS-SIY1lw as a potential antimicrobial alternative to multidrug-resistant Salmonella Infantis.Scientific reports · 2026Article
- TheJournal of virology · 2025Article
- Viral effectors trigger innate immunity across the tree of life.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- A novel Enterococcus faecalis bacteriophage Ef212: biological and genomic features.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- AeLife · 2024Article
- Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers.Nature communications · 2023Article
- AbioRxiv : the preprint server for biology · 2023Article
- A symmetry mismatch unraveled: How phage HK97 scaffold flexibly accommodates a 12-fold pore at a 5-fold viral capsid vertex.Science advances · 2023Article
- Article
- The Mottled Capsid of theViruses · 2020Article
- Capsids and Portals Influence Each Other's Conformation During Assembly and Maturation.Journal of molecular biology · 2020Article
- Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Architect of Virus Assembly: the Portal Protein Nucleates Procapsid Assembly in Bacteriophage P22.Journal of virology · 2019Article
- The Protease ClpXP and the PAS Domain Protein DivL Regulate CtrA and Gene Transfer Agent Production in Rhodobacter capsulatus.Applied and environmental microbiology · 2018Article
- "French Phage Network"-Second Meeting Report.Viruses · 2017Article
- Convergent evolution of pathogenicity islands in helper cos phage interference.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Article
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Authors and funding
5 authors.
Funding
Abstract
The 90-nm-diameter capsid of coliphage T5 is organized with T=13 icosahedral geometry and encloses a double-stranded DNA genome that measures 121kbp. Its assembly follows a path similar to that of phage HK97 but yielding a larger structure that includes 775 subunits of the major head protein, 12 subunits of the portal protein and 120 subunits of the decoration protein. As for phage HK97, T5 encodes the scaffold function as an N-terminal extension (∆-domain) to the major head protein that is cleaved by the maturation protease after assembly of the initial prohead I form and prior to DNA packaging and capsid expansion. Although the major head protein alone is sufficient to assemble capsid-like particles, the yield is poor and includes many deformed structures. Here we explore the role of both the portal and the protease in capsid assembly by generating constructs that include the major head protein and a combination of protease (wild type or an inactive mutant) and portal proteins and overexpressing them in Escherichia coli. Our results show that the inactive protease mutant acts to trigger assembly of the major head protein, probably through binding to the ∆-domain, while the portal protein regulates assembly into the correct T=13 geometry. A cryo-electron microscopy reconstruction of prohead I including inactivated protease reveals density projecting from the prohead interior surface toward its center that is compatible with the ∆-domain, as well as additional internal density that we assign as the inactivated protease. These results reveal complexity in T5 beyond that of the HK97 system.
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