Evidence map›Paper›PMID 26616285›Full record

ArticleScientific reports2015

QUANTITY: An Isobaric Tag for Quantitative Glycomics.

Shuang Yang, Meiyao Wang, Lijun Chen, Bojiao Yin, Guoqiang Song, Illarion V Turko, Karen W Phinney, Michael J Betenbaugh, Hui Zhang, Shuwei Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 72 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. 6-PlexAnalytical chemistry · 2023
    Article
  7. Article
  8. Review
  9. Review
  10. Clinically Viable Assay for Monitoring Uromodulin Glycosylation.Journal of the American Society for Mass Spectrometry · 2021
    Article
  11. Review
  12. Review
  13. Glycoengineering of Mammalian Expression Systems on a Cellular Level.Advances in biochemical engineering/biotechnology · 2021
    Article
  14. Review
  15. Article
  16. Review
  17. Molecular mechanism ofThe Journal of biological chemistry · 2019
    Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Shuang YangDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Meiyao WangInstitute for Bioscience and Biotechnology Research, University of Maryland College Park, Rockville, MD, USA.
Lijun ChenDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Bojiao YinDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Guoqiang SongSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, China.
Illarion V TurkoInstitute for Bioscience and Biotechnology Research, University of Maryland College Park, Rockville, MD, USA.
Karen W PhinneyBiomolecular Measurement Division, National Institute of Standard and Technology, Gaithersburg, MD, USA.
Michael J BetenbaughDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Hui ZhangDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Shuwei LiInstitute for Bioscience and Biotechnology Research, University of Maryland College Park, Rockville, MD, USA.
Johns Hopkins University · USUniversity of Maryland, College Park · USAltimmune (United States) · USChangzhou University · CNMaterial Measurement Laboratory · US

Funding

Shared Resource CoresP01HL107153 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HART, GERALD WARREN · 2011 to 2017
$16.8M
Proteome Characterization Center: A Genoproteomics Pipeline for Cance ResearchU24CA160036 · NCI · JOHNS HOPKINS UNIVERSITY · PI CHAN, DANIEL WANYUI, ZHANG, HUI · 2011 to 2016
$10.7M
Glycoprotein biomarkers for the early detection of aggressive prostate cancerU01CA152813 · NCI · JOHNS HOPKINS UNIVERSITY · PI ZHANG, HUI · 2010 to 2021
$6.2M
NCI NIH HHS U01 CA152813NCI NIH HHS U01CA152813NCI NIH HHS U24 CA160036NCI NIH HHS U24CA160036NHLBI NIH HHS N01-HV-00240NHLBI NIH HHS P01 HL107153NHLBI NIH HHS P01HL107153
6 · The paper itself

Abstract

Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics--analysis of glycans at global level--however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.

Indexed as

AlgorithmsAnimalsCHO CellsCricetinaeCricetulusGlycosylationMass SpectrometryMetabolomicsPolysaccharidesProteomicsReproducibility of ResultsPolysaccharides

Identifiers

PMID26616285
PMCPMC4663469
OpenAlexW2185622602

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.