ArticleScientific reports2015
A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Porcine iPSC-Derived Intestinal Cells as a High-Fidelity Platform for PEDV Pathogenesis.Animals : an open access journal from MDPI · 2026Article
- Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation.Biomedical optics express · 2024Article
- Interactions of SARS-CoV-2 with Human Target Cells-A Metabolic View.International journal of molecular sciences · 2024Review
- Accessible luminal interface of bovine rectal organoids generated from cryopreserved biopsy tissues.PloS one · 2024Article
- SARS-CoV-2 induces barrier damage and inflammatory responses in the human iPSC-derived intestinal epithelium.Journal of pharmacological sciences · 2022Review
- Characterization and reduction of non-endocrine cells accompanying islet-like endocrine cells differentiated from human iPSC.Scientific reports · 2022Article
- Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells.International journal of molecular sciences · 2022Article
- The development of a functional human small intestinal epithelium model for drug absorption.Science advances · 2021Article
- Article
- Vinblastine treatment decreases the undifferentiated cell contamination of human iPSC-derived intestinal epithelial-like cells.Molecular therapy. Methods & clinical development · 2021Article
- Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system.Scientific reports · 2021Article
- Generation of Human-Induced Pluripotent Stem Cell-Derived Functional Enterocyte-Like Cells for Pharmacokinetic Studies.Stem cell reports · 2021Article
- Human small intestinal organotypic culture model for drug permeation, inflammation, and toxicity assays.In vitro cellular & developmental biology. Animal · 2021Review
- Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells.International journal of molecular sciences · 2020Article
- Establishment of a novel culture method for maintaining intestinal stem cells derived from human induced pluripotent stem cells.Biology open · 2020Article
- Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies.Stem cell reports · 2018Article
- A Simple System for Differentiation of Functional Intestinal Stem Cell-like Cells from Bone Marrow Mesenchymal Stem Cells.Molecular therapy. Nucleic acids · 2018Article
- Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells.PloS one · 2018Article
- A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family.PloS one · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells. In the presence of dimethyl sulfoxide (DMSO), a low concentration of Activin at 6.25 ng/ml is sufficient to give a similar differentiation efficiency with that using Activin at 100 ng/ml at the presence of Wnt activator. In the presence of DMSO, Activin at low concentration triggered hiPS cells to undergo differentiation through G1 arrest, reduce apoptosis, and potentiate activation of downstream targets, such as SMAD2 phosphorylation and SOX17 expression. This increased differentiation into CDX2 + SOX17 + DE cells. The present differentiation procedure therefore permits rapid and efficient derivation of DE cells, capable of differentiating into intestinal epithelium upon BIO and DAPT treatment and of giving rise to functional cells, such as enterocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.