ArticleThe Journal of veterinary medical science2016
Development of a novel detection system for microbes from bovine diarrhea by real-time PCR.
Article in The Journal of veterinary medical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 35 citations in OpenAlex.
- Diagnostic Methods for Bovine Coronavirus: A Review of Recent Advancements and Challenges.Viruses · 2025Review
- Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines.The Journal of veterinary medical science · 2025Article
- Effect of pre-weaning glycyl-glutamine feeding on diarrhea rate, hindgut fermentation, antioxidant stress capability, and fecal microbiota in calves.Journal of animal science · 2025Article
- Maternal Transmission of Rotavirus to Calves and Comparison of Colostrum and Fecal Microbiota in Holstein and Hanwoo Cattle.Veterinary sciences · 2024Article
- Identification of multiple inter- and intra-genotype reassortment mammalian orthoreoviruses from Japanese black cattle in a beef cattle farm.Scientific reports · 2024Article
- Article
- Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology.Frontiers in veterinary science · 2024Article
- Analysis of Fecal Microbial Changes in Young Calves Following Bovine Rotavirus Infection.Veterinary sciences · 2023Article
- Antisera Produced Using anInternational journal of molecular sciences · 2023Article
- Development of a one-run real-time PCR detection system for pathogens associated with poultry infectious diseases.The Journal of veterinary medical science · 2023Article
- AnInternational journal of molecular sciences · 2022Article
- Efficiency-corrected PCR quantification for identification of prevalence and load of respiratory disease-causing agents in feedlot cattle.Australian veterinary journal · 2022Article
- Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea.Journal of applied microbiology · 2022Article
- Rapid detection of bovine rotavirus a by isothermal reverse transcription recombinase polymerase amplification assays.BMC veterinary research · 2022Article
- Development of a multienzyme isothermal rapid amplification and lateral flow dipstick combination assay for bovine coronavirus detection.Frontiers in veterinary science · 2022Article
- Development of a One-Step Multiplex Real-Time PCR Assay for the Detection of Viral Pathogens Associated With the Bovine Respiratory Disease Complex.Frontiers in veterinary science · 2022Article
- Broad detection and quick differentiation of bovine viral diarrhea viruses 1 and 2 by a reverse transcription loop-mediated isothermal amplification test.The Journal of veterinary medical science · 2021Article
- Article
- Longitudinal evaluation of fecal microbiota transplantation for ameliorating calf diarrhea and improving growth performance.Nature communications · 2021Article
- Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diarrhea in cattle is one of the most economically costly disorders, decreasing milk production and weight gain. In the present study, we established a novel simultaneous detection system using TaqMan real-time PCR designed as a system for detection of microbes from bovine diarrhea using real-time PCR (referred to as Dembo-PCR). Dembo-PCR simultaneously detects a total of 19 diarrhea-causing pathogens, including viruses, bacteria and protozoa. Specific primer-probe sets were newly designed for 7 pathogens and were synthesized on the basis of previous reports for 12 pathogens. Assays were optimized to react under the same reaction conditions. The PCR efficiency and correlation coefficient (R(2)) of standard curves for each assay were more than 80% and 0.9766, respectively. Furthermore, the sensitivity of Dembo-PCR in fecal sample analysis was measured with feces spiked with target pathogens or synthesized DNA that included specific nucleotide target regions. The resulting limits of detection (LOD) for virus-spiked samples, bacteria and DNA fragments were 0.16-1.6 TCID50 (PFU/reaction), 1.3-13 CFU/reaction and 10-100 copies/reaction, respectively. All reactions showed high sensitivity in pathogen detection. A total of 8 fecal samples, collected from 6 diarrheic cattle, 1 diarrheic calf and 1 healthy cow, were tested using Dembo-PCR to validate the assay's clinical performance. The results revealed that bovine coronavirus had infected all diarrheic adult cattle and that bovine torovirus had infected the diarrheic calf. These results suggest that Dembo-PCR may be a powerful tool for diagnosing infectious agents in cattle diarrhea.
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