ArticleScientific reports2015
The power of single molecule real-time sequencing technology in the de novo assembly of a eukaryotic genome.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Comparative genomics and transcriptomics on salt tolerance ofBreeding science · 2026Article
- High-Resolution Genomic Resources for Trait Mapping and Precision Breeding for Adzuki Bean (Vigna angularis).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Diurnal Regulation of SOS Pathway and Sodium Excretion Underlying Salinity Tolerance of Vigna marina.Plant, cell & environment · 2025Article
- Chromosome-level assembly ofBreeding science · 2025Article
- The genomic landscape of gene-level structural variations in Japanese and global soybean Glycine max cultivars.Nature genetics · 2025Article
- A special short-wing petal faba genome and genetic dissection of floral and yield-related traits accelerate breeding and improvement of faba bean.Genome biology · 2025Article
- Chromosome genome assembly and annotation of Adzuki Bean (Vigna angularis).Scientific data · 2024Article
- Taxonomic history, morphology, evolution, gene pool and stress tolerances of azuki bean and its related wildBreeding science · 2024Article
- Single candidate gene for salt tolerance ofBreeding science · 2024Article
- A view of the pan-genome of domesticated Cowpea (Vigna unguiculata [L.] Walp.).The plant genome · 2024Article
- Translocations and inversions: major chromosomal rearrangements during Vigna (Leguminosae) evolution.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Unique Salt-Tolerance-Related QTLs, Evolved inPlants (Basel, Switzerland) · 2023Article
- Mining legume germplasm for genetic gains: An Indian perspective.Frontiers in genetics · 2023Review
- Advancing Grain Legumes Domestication and Evolution Studies with Genomics.Plant & cell physiology · 2022Review
- Cantaloupe melon genome reveals 3D chromatin features and structural relationship with the ancestral cucurbitaceae karyotype.iScience · 2022Article
- Thirty Years of Mungbean Genome Research: Where Do We Stand and What Have We Learned?Frontiers in plant science · 2022Review
- BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris.Chromosoma · 2021Article
- Unequal contribution of two paralogous CENH3 variants in cowpea centromere function.Communications biology · 2020Article
- Breaks of macrosynteny and collinearity among moth bean (Vigna aconitifolia), cowpea (V. unguiculata), and common bean (Phaseolus vulgaris).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- Comparative genomics of muskmelon reveals a potential role for retrotransposons in the modification of gene expression.Communications biology · 2020Article
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14 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Second-generation sequencers (SGS) have been game-changing, achieving cost-effective whole genome sequencing in many non-model organisms. However, a large portion of the genomes still remains unassembled. We reconstructed azuki bean (Vigna angularis) genome using single molecule real-time (SMRT) sequencing technology and achieved the best contiguity and coverage among currently assembled legume crops. The SMRT-based assembly produced 100 times longer contigs with 100 times smaller amount of gaps compared to the SGS-based assemblies. A detailed comparison between the assemblies revealed that the SMRT-based assembly enabled a more comprehensive gene annotation than the SGS-based assemblies where thousands of genes were missing or fragmented. A chromosome-scale assembly was generated based on the high-density genetic map, covering 86% of the azuki bean genome. We demonstrated that SMRT technology, though still needed support of SGS data, achieved a near-complete assembly of a eukaryotic genome.
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