ArticleGenetics2016
Centromere Locations in Brassica A and C Genomes Revealed Through Half-Tetrad Analysis.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of seed weight QTLome using a consensus and highly dense genetic map in Brassica napus L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Pooled it
- Synthetic Raphanobrassica Genome Reveals Functional and Evolutionary Insights Into Clubroot Resistance Genes on Chromosome R5.Plant biotechnology journal · 2026Article
- Machine learning and multi-omic analysis reveal contrasting recombination landscape of A and C subgenomes of winter oilseed rape.The plant genome · 2026Article
- Substantial non-homologous recombination and structural variation results from Brassica AABC and CCAB hybrid meiosis.The Plant journal : for cell and molecular biology · 2025Article
- Genomic Divergence Shaped the Genetic Regulation of Meiotic Homologous Recombination in Brassica Allopolyploids.Molecular biology and evolution · 2025Article
- Structural variation reshapes population gene expression and trait variation in 2,105 Brassica napus accessions.Nature genetics · 2024Article
- Article
- Heterozygosity analysis of spontaneous 2n female gametes and centromere mapping of the diploid Hevea brasiliensis based on full-sib triploid populations.Plant reproduction · 2024Article
- Genome composition in Brassica interspecific hybrids affects chromosome inheritance and viability of progeny.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
- Genome sequence analysis provides insights into the mode of 2n egg formation in Solanum malmeanum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Novel gene loci associated with susceptibility or cryptic quantitative resistance to Pyrenopeziza brassicae in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- R gene triplication confers European fodder turnip with improved clubroot resistance.Plant biotechnology journal · 2022Article
- Frequent spontaneous structural rearrangements promote rapid genome diversification in aFrontiers in plant science · 2022Article
- Modelling of gene loss propensity in the pangenomes of three Brassica species suggests different mechanisms between polyploids and diploids.Plant biotechnology journal · 2021Article
- A Modified Meiotic Recombination inBiology · 2021Article
- Enhancing backcross programs through increased recombination.Genetics, selection, evolution : GSE · 2021Article
- Long-read assembly of the Brassica napus reference genome Darmor-bzh.GigaScience · 2020Article
- Localization and characterization of Citrus centromeres by combining half-tetrad analysis and CenH3-associated sequence profiling.Plant cell reports · 2020Article
- Assessing by Modeling the Consequences of Increased Recombination in Recurrent Selection ofG3 (Bethesda, Md.) · 2019Article
- Genetic changes in a novel breeding population of Brassica napus synthesized from hundreds of crosses between B. rapa and B. carinata.Plant biotechnology journal · 2018Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Locating centromeres on genome sequences can be challenging. The high density of repetitive elements in these regions makes sequence assembly problematic, especially when using short-read sequencing technologies. It can also be difficult to distinguish between active and recently extinct centromeres through sequence analysis. An effective solution is to identify genetically active centromeres (functional in meiosis) by half-tetrad analysis. This genetic approach involves detecting heterozygosity along chromosomes in segregating populations derived from gametes (half-tetrads). Unreduced gametes produced by first division restitution mechanisms comprise complete sets of nonsister chromatids. Along these chromatids, heterozygosity is maximal at the centromeres, and homologous recombination events result in homozygosity toward the telomeres. We genotyped populations of half-tetrad-derived individuals (from Brassica interspecific hybrids) using a high-density array of physically anchored SNP markers (Illumina Brassica 60K Infinium array). Mapping the distribution of heterozygosity in these half-tetrad individuals allowed the genetic mapping of all 19 centromeres of the Brassica A and C genomes to the reference Brassica napus genome. Gene and transposable element density across the B. napus genome were also assessed and corresponded well to previously reported genetic map positions. Known centromere-specific sequences were located in the reference genome, but mostly matched unanchored sequences, suggesting that the core centromeric regions may not yet be assembled into the pseudochromosomes of the reference genome. The increasing availability of genetic markers physically anchored to reference genomes greatly simplifies the genetic and physical mapping of centromeres using half-tetrad analysis. We discuss possible applications of this approach, including in species where half-tetrads are currently difficult to isolate.
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