Evidence map›Paper›PMID 26613341›Full record

ArticleThe ISME journal2016

High levels of heterogeneity in diazotroph diversity and activity within a putative hotspot for marine nitrogen fixation.

Lauren F Messer, Claire Mahaffey, Charlotte M Robinson, Thomas C Jeffries, Kirralee G Baker, Jaime Bibiloni Isaksson, Martin Ostrowski, Martina A Doblin, Mark V Brown, Justin R Seymour

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
9.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 69 citations in OpenAlex.

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  3. Size-fractionated NFrontiers in microbiology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Lauren F MesserPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Claire MahaffeyDepartment of Earth, Ocean and Ecological Sciences, University of Liverpool, Liverpool, UK.
Charlotte M RobinsonPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Thomas C JeffriesPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Kirralee G BakerPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Jaime Bibiloni IsakssonPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Martin OstrowskiDepartment of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, New South Wales, Australia.
Martina A DoblinPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Mark V BrownSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
Justin R SeymourPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
University of Technology Sydney · AUMacquarie University · AUUniversity of Liverpool · GBUNSW Sydney · AUWestern Sydney University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Australia's tropical waters represent predicted 'hotspots' for nitrogen (N2) fixation based on empirical and modelled data. However, the identity, activity and ecology of diazotrophs within this region are virtually unknown. By coupling DNA and cDNA sequencing of nitrogenase genes (nifH) with size-fractionated N2 fixation rate measurements, we elucidated diazotroph dynamics across the shelf region of the Arafura and Timor Seas (ATS) and oceanic Coral Sea during Austral spring and winter. During spring, Trichodesmium dominated ATS assemblages, comprising 60% of nifH DNA sequences, while Candidatus Atelocyanobacterium thalassa (UCYN-A) comprised 42% in the Coral Sea. In contrast, during winter the relative abundance of heterotrophic unicellular diazotrophs (δ-proteobacteria and γ-24774A11) increased in both regions, concomitant with a marked decline in UCYN-A sequences, whereby this clade effectively disappeared in the Coral Sea. Conservative estimates of N2 fixation rates ranged from <1 to 91 nmol l(-1) day(-1), and size fractionation indicated that unicellular organisms dominated N2 fixation during both spring and winter, but average unicellular rates were up to 10-fold higher in winter than in spring. Relative abundances of UCYN-A1 and γ-24774A11 nifH transcripts negatively correlated to silicate and phosphate, suggesting an affinity for oligotrophy. Our results indicate that Australia's tropical waters are indeed hotspots for N2 fixation and that regional physicochemical characteristics drive differential contributions of cyanobacterial and heterotrophic phylotypes to N2 fixation.

Indexed as

CyanobacteriaDeltaproteobacteriaAnimalsAnthozoaAustraliaEcologyHeterotrophic ProcessesNitrogenaseNitrogen FixationOceans and SeasSeasonsSeawaterSequence Analysis, DNATrichodesmiumNitrogenase

Identifiers

PMID26613341
PMCPMC5029180
OpenAlexW2177730169

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.