ArticleBiotechnology for biofuels2015
Single-cell screening of photosynthetic growth and lactate production by cyanobacteria.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells.Nature communications · 2025Article
- From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.Biomicrofluidics · 2025Article
- Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing.Synthetic and systems biotechnology · 2023Article
- Droplets microfluidics platform-A tool for single cell research.Frontiers in bioengineering and biotechnology · 2023Review
- Lab-scale photobioreactor systems: principles, applications, and scalability.Bioprocess and biosystems engineering · 2022Review
- Droplet Microfluidics for Microbial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- Microfluidic Single-Cell Analytics.Advances in biochemical engineering/biotechnology · 2022Article
- Method for Passive Droplet Sorting after Photo-Tagging.Micromachines · 2020Article
- Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes.Nature communications · 2020Article
- Optofluidic detection setup for multi-parametric analysis of microbiological samples in droplets.Biomicrofluidics · 2020Article
- N-Substituted Auxiliaries for Aerobic Dehydrogenation of Tetrahydro-isoquinoline: A Theory-Guided Photo-Catalytic Design.Scientific reports · 2019Article
- Beyond the bulk: disclosing the life of single microbial cells.FEMS microbiology reviews · 2017Review
- Review of Microfluidic Photobioreactor Technology for Metabolic Engineering and Synthetic Biology of Cyanobacteria and Microalgae.Micromachines · 2016Review
- Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis.Molecules (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhotosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible.
resultsWe present a method for high-throughput, single-cell analysis and sorting of genetically engineered l-lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic device is used to encapsulate single cells in picoliter droplets, assay the droplets for l-lactate production, and sort strains with high productivity. We demonstrate the separation of low- and high-producing reference strains, as well as enrichment of a more productive l-lactate-synthesizing population after UV-induced mutagenesis. The droplet platform also revealed population heterogeneity in photosynthetic growth and lactate production, as well as the presence of metabolically stalled cells.
conclusionsThe workflow will facilitate metabolic engineering and directed evolution studies and will be useful in studies of cyanobacteria biochemistry and physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.