ArticleStem cell research & therapy2015
Cryopreservation of equine mesenchymal stem cells in 95% autologous serum and 5% DMSO does not alter post-thaw growth or morphology in vitro compared to fetal bovine serum or allogeneic serum at 20 or 95% and DMSO at 10 or 5.
Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review.Journal of translational medicine · 2019Pooled it
- Addition of synthetic polymer in the freezing solution of mesenchymal stem cells from equine adipose tissue as a future perspective for reducing of DMSO concentration.Brazilian journal of veterinary medicine · 2023Article
- Cross-matching of allogeneic mesenchymal stromal cells eliminates recipient immune targeting.Stem cells translational medicine · 2021Article
- The Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism.Stem cells international · 2021Article
- Preparation Technique Affects Recipient Immune Targeting of Autologous Mesenchymal Stem Cells.Frontiers in veterinary science · 2021Article
- Quantitative assessment of the impact of cryopreservation on human bone marrow-derived mesenchymal stem cells: up to 24 h post-thaw and beyond.Stem cell research & therapy · 2020Article
- Downregulation of type I collagen expression in the Achilles tendon by dexamethasone: a controlled laboratory study.Journal of orthopaedic surgery and research · 2020Article
- Multidose intramuscular allogeneic adipose stem cells decrease the severity of canine atopic dermatitis: A pilot study.Veterinary world · 2019Article
- Effect of intra-ovarian injection of mesenchymal stem cells in aged mares.Journal of assisted reproduction and genetics · 2019Article
- Persistence of fluorescent nanoparticle-labelled bone marrow mesenchymal stem cells in vitro and after intra-articular injection.Journal of tissue engineering and regenerative medicine · 2019Article
- Evaluation of three cryoprotectants used with bovine milk affected with Mycoplasma bovis in different freezing conditions.BMC research notes · 2018Article
- Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers.Journal of veterinary science · 2018Article
- Adult multipotent stromal cell cryopreservation: Pluses and pitfalls.Veterinary surgery : VS · 2018Review
- Repeated intra-articular injection of allogeneic mesenchymal stem cells causes an adverse response compared to autologous cells in the equine model.Stem cell research & therapy · 2017Article
- Article
- Optimised cryopreservation preserves functional competence of goat adipose-derived mesenchymal stem cells and is associated with stress-adapted mitochondrial and paracrine features.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionEquine superficial digital flexor tendon injury is a well-accepted model of human tendon injury and is routinely treated with local injections of autologous mesenchymal stem cells (MSCs). Identification of a clinically safe medium for short-term cryopreservation of MSCs prior to cell implantation would streamline laboratory and clinical procedures for autologous regenerative therapies. Veterinary experience with short-term (MSCs prepared after the injury has occurred) cryopreserved MSCs in naturally occurring injury in the horse will be of value to human practitioners.
methodsEquine bone marrow derived MSCs were cryopreserved in 6 different solutions consisting of 20% serum, 10% DMSO and 70% media or 95% serum and 5% DMSO. Serum was autologous serum, commercially available pooled equine serum or fetal bovine serum (FBS). Cell survival, morphology and growth kinetics were assessed by total cell number, measurement of growth kinetics, colony-forming-unit-assay and morphology of MSCs after monolayer culture post-thaw.
resultsThere were no significant differences in post-thaw viability, total cell number, morphology scores or growth kinetics among the 6 solutions. Post thaw viabilities from each group ranged from 80-90%. In all solutions, there were significantly fewer MSCs and the majority (99%) of MSCs remained in the original generation 24 hours post-thaw. Seventy two hours post-thaw, the majority of MSCs (50%) were proliferating in the fourth generation. Mean colony count in the CFU-F assay ranged from 72 to 115 colonies.
conclusionsEach of the serum sources could be used for short-term cryopreservation of equine bone marrow derived MSCs. Prior to clinical use, clinicians may prefer autologous serum and a lower concentration of DMSO.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.