Evidence map›Paper›PMID 26611747›Full record

ArticleThe Journal of thoracic and cardiovascular surgery2016

Characterizing the angiogenic activity of patients with single ventricle physiology and aortopulmonary collateral vessels.

Nefthi Sandeep, Yutaka Uchida, Kanishka Ratnayaka, Robert McCarter, Sridhar Hanumanthaiah, Aminata Bangoura, Zhen Zhao, Jacqueline Oliver-Danna, Linda Leatherbury, Joshua Kanter and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of thoracic and cardiovascular surgery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Molecular Approaches in Single Ventricle Management.Seminars in thoracic and cardiovascular surgery. Pediatric cardiac surgery annual · 2020
    Review
  7. Observational
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Nefthi SandeepLaboratory of Stem Cell and Neurovascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md; Division of Pediatric Cardiology, Children's National Health System, Washington, DC.
Yutaka UchidaLaboratory of Stem Cell and Neurovascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md.
Kanishka RatnayakaDivision of Pediatric Cardiology, Children's National Health System, Washington, DC.
Robert McCarterDepartment of Biostatistics & Informatics, Children's National Health System, Washington, DC.
Sridhar HanumanthaiahDivision of Pediatric Cardiology, Children's National Health System, Washington, DC.
Aminata BangouraDivision of Pediatric Cardiology, Children's National Health System, Washington, DC.
Zhen ZhaoDepartment of Laboratory Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md.
Jacqueline Oliver-DannaDepartment of Laboratory Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md.
Linda LeatherburyDivision of Pediatric Cardiology, Children's National Health System, Washington, DC.
Joshua KanterDivision of Pediatric Cardiology, Children's National Health System, Washington, DC.
Yoh-Suke MukouyamaLaboratory of Stem Cell and Neurovascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md. Electronic address: mukoyamay@mail.nih.gov.
Children's National · USNational Institutes of Health · USNational Heart Lung and Blood Institute · US

Funding

Clinical and Translational Science Institute at Children's NationalUL1TR000075 · NCATS · CHILDREN'S RESEARCH INSTITUTE · PI GUAY-WOODFORD, LISA MARIE · 2012 to 2015
$13.7M
The study of neuro-vascular interactions in the developing heartZIAHL006116 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI MUKOUYAMA, YOH-SUKE · 2011 to 2025
$9.4M
Intramural NIH HHS ZIA HL006116NCATS NIH HHS UL1 TR000075NCATS NIH HHS UL1TR000075NHLBI NIH HHS HL006116-05
6 · The paper itself

Abstract

objectivesPatients with single ventricle congenital heart disease often form aortopulmonary collateral vessels via an unclear mechanism. To gain insights into the pathogenesis of aortopulmonary collateral vessels, we correlated angiogenic factor levels with in vitro activity and angiographic aortopulmonary collateral assessment and examined whether patients with single ventricle physiology have increased angiogenic factors that can stimulate endothelial cell sprouting in vitro.

methodsIn patients with single ventricle physiology (n = 27) and biventricular acyanotic control patients (n = 21), hypoxia-inducible angiogenic factor levels were measured in femoral venous and arterial plasma at cardiac catheterization. To assess plasma angiogenic activity, we used a 3-dimensional in vitro cell sprouting assay that recapitulates angiogenic sprouting. Aortopulmonary collateral angiograms were graded using a 4-point scale.

resultsCompared with controls, patients with single ventricle physiology had increased vascular endothelial growth factor (artery: 58.7 ± 1.2 pg/mL vs 35.3 ± 1.1 pg/mL, P < .01; vein: 34.8 ± 1.1 pg/mL vs 21 ± 1.2 pg/mL, P < .03), stromal-derived factor 1-alpha (artery: 1901.6 ± 1.1 pg/mL vs 1542.6 ± 1.1 pg/mL, P < .03; vein: 2092.8 pg/mL ± 1.1 vs 1752.9 ± 1.1 pg/mL, P < .02), and increased arterial soluble fms-like tyrosine kinase-1, a regulatory vascular endothelial growth factor receptor (612.3 ± 1.2 pg/mL vs 243.1 ± 1.2 pg/mL, P < .003). Plasma factors and sprout formation correlated poorly with aortopulmonary collateral severity.

conclusionsWe are the first to correlate plasma angiogenic factor levels with angiography and in vitro angiogenic activity in patients with single ventricle disease with aortopulmonary collaterals. Patients with single ventricle disease have increased stromal-derived factor 1-alpha and soluble fms-like tyrosine kinase-1, and their roles in aortopulmonary collateral formation require further investigation. Plasma factors and angiogenic activity correlate poorly with aortopulmonary collateral severity in patients with single ventricles, suggesting complex mechanisms of angiogenesis.

Indexed as

Collateral CirculationNeovascularization, PhysiologicPulmonary CirculationAdolescentAngiogenic ProteinsAortaAortographyCase-Control StudiesCells, CulturedChemokine CXCL12ChildChild, PreschoolEndothelial CellsFemaleHeart Defects, CongenitalHumansAngiogenic ProteinsChemokine CXCL12CXCL12 protein, humanVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2VEGFA protein, humanaortopulmonary collateralcongenital heart diseasesingle ventricle

Identifiers

PMID26611747
PMCPMC4801745
OpenAlexW1958080898

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.