Evidence map›Paper›PMID 26611444›Full record

ArticleScientific reports2015

ω-Tbo-IT1-New Inhibitor of Insect Calcium Channels Isolated from Spider Venom.

Alexander N Mikov, Irina M Fedorova, Natalia N Potapieva, Ekaterina E Maleeva, Yaroslav A Andreev, Alexey V Zaitsev, Kira K Kim, Eduard V Bocharov, Timur N Bozin, Dmitry A Altukhov and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Alexander N MikovDepartment of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia.
Irina M FedorovaLaboratory of Biophysics of Synaptic Processes, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, Russia.
Natalia N PotapievaLaboratory of Biophysics of Synaptic Processes, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, Russia.
Ekaterina E MaleevaDepartment of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia.
Yaroslav A AndreevDepartment of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia.
Alexey V ZaitsevLaboratory of Biophysics of Synaptic Processes, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, Russia.
Kira K KimLaboratory of Biophysics of Synaptic Processes, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, Russia.
Eduard V BocharovDepartment of Structural Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia.
Timur N BozinNBIC Centre, NRC "Kurchatov Institute", Russia.
Dmitry A AltukhovNBIC Centre, NRC "Kurchatov Institute", Russia.
Alexey V LipkinNBIC Centre, NRC "Kurchatov Institute", Russia.
Sergey A KozlovDepartment of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia.
Denis B TikhonovLaboratory of Biophysics of Synaptic Processes, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, Russia.
Eugene V GrishinDepartment of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Russia.
Institute of Bioorganic Chemistry · RUInstitute of Evolutionary Physiology and Biochemistry · RUKurchatov Institute · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Novel disulfide-containing polypeptide toxin was discovered in the venom of the Tibellus oblongus spider. We report on isolation, spatial structure determination and electrophysiological characterization of this 41-residue toxin, called ω-Tbo-IT1. It has an insect-toxic effect with LD50 19 μg/g in experiments on house fly Musca domestica larvae and with LD50 20 μg/g on juvenile Gromphadorhina portentosa cockroaches. Electrophysiological experiments revealed a reversible inhibition of evoked excitatory postsynaptic currents in blow fly Calliphora vicina neuromuscular junctions, while parameters of spontaneous ones were not affected. The inhibition was concentration dependent, with IC50 value 40 ± 10 nM and Hill coefficient 3.4 ± 0.3. The toxin did not affect frog neuromuscular junctions or glutamatergic and GABAergic transmission in rat brains. Ca(2+) currents in Calliphora vicina muscle were not inhibited, whereas in Periplaneta americana cockroach neurons at least one type of voltage gated Ca(2+) current was inhibited by ω-Tbo-IT1. Thus, the toxin apparently acts as an inhibitor of presynaptic insect Ca(2+) channels. Spatial structure analysis of the recombinant ω-Tbo-IT1 by NMR spectroscopy in aqueous solution revealed that the toxin comprises the conventional ICK fold containing an extended β-hairpin loop and short β-hairpin loop which are capable of making "scissors-like mutual motions".

Indexed as

Amino Acid SequenceAnimalsAnuraCalciumCalcium Channel BlockersCalcium ChannelsCells, CulturedCloning, MolecularCockroachesDipteraEscherichia coliExcitatory Postsynaptic PotentialsGene ExpressionInsect ProteinsLarvaModels, MolecularCalciumCalcium Channel BlockersCalcium ChannelsInsect ProteinsRecombinant ProteinsSpider Venoms

Identifiers

PMID26611444
PMCPMC4661699
OpenAlexW2418598139

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.