Evidence map›Paper›PMID 26611228›Full record

ArticleJournal of virological methods2016

Consistent viral DNA quantification after prolonged storage at ambient temperature.

Benjamin Zaniello, Meei-Li Huang, Anqi Cheng, Stacy Selke, Anna Wald, Keith R Jerome, Amalia S Magaret

Abstract read
In one paragraph

Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Stability of SpikedOpen forum infectious diseases · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Benjamin ZanielloDepartment of Medicine, University of Washington, United States.
Meei-Li HuangVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, United States; Department of Laboratory Medicine, University of Washington, United States.
Anqi ChengDepartment of Biostatistics, University of Washington, United States.
Stacy SelkeDepartment of Laboratory Medicine, University of Washington, United States.
Anna WaldDepartment of Medicine, University of Washington, United States; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, United States; Department of Laboratory Medicine, University of Washington, United States; Department of Epidemiology, University of Washington, United States.
Keith R JeromeVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, United States; Department of Laboratory Medicine, University of Washington, United States.
Amalia S MagaretVaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, United States; Department of Laboratory Medicine, University of Washington, United States; Department of Biostatistics, University of Washington, United States. Electronic address: amag@uw.edu.

Funding

THE PERINATAL COMPLICATIONS OF ASYMPTOMATIC GENITAL HERPES SIMPLEX VIRUSP01AI030731 · NIAID · UNIVERSITY OF WASHINGTON · PI MAGARET, AMALIA S. · 1991 to 2017
$34.3M
HOST DEFENSE TRAINING IN ALLERGY AND INFECTIOUS DISEASEST32AI007044 · NIAID · UNIVERSITY OF WASHINGTON · PI DAVID Neal FREDRICKS · 1985 to 2026
$14.6M
Clinical Research in HSV InfectionsK24AI071113 · NIAID · UNIVERSITY OF WASHINGTON · PI WALD, ANNA · 2006 to 2015
$1.7M
NIAID NIH HHS K24 AI 071113NIAID NIH HHS K24 AI071113NIAID NIH HHS P01 AI 030731NIAID NIH HHS P01 AI030731NIAID NIH HHS T32 AI007044NIAID NIH HHS T32 AI 007044-37
6 · The paper itself

Abstract

Long-term storage of biological specimens at low temperatures is costly and impractical in resource limited settings, where the disease burden of chronic viral infections is highest, and the need for research greatest. We examined the necessity of cold storage by comparing the quantity of HHV-8 DNA recovered from swab samples before and after 9-11 months of storage at temperatures of -20 °C, 4 °C and 37 °C. Quantitative levels of HHV-8 DNA remained consistent for laboratory or mucosal swab samples regardless of storage temperature. Freezer storage is determined to be not necessary for mucosal samples destined for HHV-8 DNA quantification.

Indexed as

CryopreservationMicrobial ViabilityTemperatureDNA, ViralHerpesviridae InfectionsHerpesvirus 8, HumanHumansMucous MembraneReal-Time Polymerase Chain ReactionSpecimen HandlingDNA, ViralFreezingHHV-8Real-time PCRRoom temperatureStorage

Identifiers

PMID26611228
PMCPMC4718791

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.