Evidence map›Paper›PMID 26608594›Full record

ArticleAllergy2016

Mosquito salivary allergen Aed a 3: cloning, comprehensive molecular analysis, and clinical evaluation.

Z Peng, W W Xu, Y Sham, H Lam, D Sun, L Cheng, N F Rasic, Q Guan, A A James, F E R Simons

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Allergy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Frontiers in microbiology · 2019
    Review
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Z PengDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
W W XuDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
Y ShamCenter for Drug Design, Academic Health Center, University of Minnesota, Minneapolis, MN, USA.
H LamDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
D SunDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
L ChengDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
N F RasicDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
Q GuanDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
A A JamesDepartments of Microbiology & Molecular Genetics and Molecular Biology & Biochemistry, University of California, Irvine, CA, USA.
F E R SimonsDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
University of Manitoba · CAUniversity of California, Irvine · USUniversity of Minnesota · US

Funding

Expression of Exogenous Genes in Vector MosquitoesR37AI029746 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI JAMES, ANTHONY A. · 2007 to 2016
$3.6M
EXPRESSION OF EXOGENOUS GENES IN VECTOR MOSQUITOESR01AI029746 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI JAMES, ANTHONY A. · 1989 to 2006
$1.7M
NIAID NIH HHS R01 AI029746NIAID NIH HHS R37 AI029746
6 · The paper itself

Abstract

backgroundAllergic reactions to mosquito bites are an increasing clinical concern. Due to the lack of availability of mosquito salivary allergens, they are underdiagnosed. Here, we reported a newly cloned mosquito Aedes (Ae.) aegypti salivary allergen.

methodsA cDNA encoding a 30-kDa Ae. aegypti salivary protein, designated Aed a 3, was isolated from an expression library. The full-length cDNA was cloned into a baculovirus expression vector, and recombinant Aed a 3 (rAed a 3) was expressed, purified, and characterized. Skin prick tests with purified rAed a 3 and Ae. aegypti bite tests were performed in 43 volunteers. Serum rAed a 3-specific IgE levels were measured in 28 volunteers.

resultsThe primary nucleotide sequence, deduced amino acid sequence, and IgE-binding sites of Aed a 3 were identified. rAed a 3-selected antibodies recognized a 30-kDa Ae. aegypti saliva protein. rAed a 3 bound IgE in mosquito-allergic volunteers and the binding could be inhibited by the addition of natural mosquito extract dose dependently. Immediate skin test reactions to rAed a 3 correlated significantly with mosquito bite-induced reactions. Of the bite test-positive volunteers, 32% had a positive rAed a 3 skin test and 46% had specific IgE. No bite test-negative volunteers reacted to rAed a 3 in either the skin tests or the IgE assays, confirming the specificity of the assay.

conclusionsAed a 3 that corresponds to the Aegyptin protein is a major mosquito salivary allergen. Its recombinant form has biological activity and is suitable for use in skin tests and specific IgE assays in mosquito-allergic individuals.

Indexed as

AdultAedesAllergensAmino Acid SequenceAnimalsCloning, MolecularDNA, ComplementaryEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin EInsect Bites and StingsInsect ProteinsModels, MolecularProtein BindingProtein ConformationAllergensDNA, ComplementaryImmunoglobulin EInsect ProteinsRecombinant ProteinsSalivary Proteins and PeptidesAed a 3Aedes aegyptiinsect allergymosquitosalivary allergen/antigen

Identifiers

PMID26608594
PMCPMC4844854
OpenAlexW2178828030

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.