ArticleScientific reports2015
Time-lapse electrical impedance spectroscopy for monitoring the cell cycle of single immobilized S. pombe cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Integrated Microfluidics for Single-Cell Separation and On-Chip Analysis: Novel Applications and Recent Advances.Small science · 2024Article
- Three-dimensional topology-based analysis segments volumetric and spatiotemporal fluorescence microscopy.Biological imaging · 2024Article
- Impedance Imaging of Cells and Tissues: Design and Applications.BME frontiers · 2022Article
- A high-throughput microfluidic diploid yeast long-term culturing (DYLC) chip capable of bud reorientation and concerted daughter dissection for replicative lifespan determination.Journal of nanobiotechnology · 2022Article
- Analytics in Microfluidic Systems.Advances in biochemical engineering/biotechnology · 2022Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research.Advanced healthcare materials · 2021Review
- Investigation on the Direct and Bystander Effects in HeLa Cells Exposed to Very Low α-Radiation Using Electrical Impedance Measurement.ACS omega · 2021Article
- Real-Time Monitoring of Dissection Events of Single Budding Yeast in a Microfluidic Cell-Culturing Device Integrated With Electrical Impedance Biosensor.Frontiers in bioengineering and biotechnology · 2021Article
- Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells.Analytical and bioanalytical chemistry · 2020Article
- Microfluidic Single-Cell Manipulation and Analysis: Methods and Applications.Micromachines · 2019Review
- Developments in label-free microfluidic methods for single-cell analysis and sorting.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems.ACS chemical biology · 2018Review
- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo.Methods in molecular biology (Clifton, N.J.) · 2018Review
- A microfluidic platform for drug screening in a 3D cancer microenvironment.Biosensors & bioelectronics · 2017Article
- Recent Advances in the Analysis of Single Cells.Analytical chemistry · 2017Review
- A Versatile Bonding Method for PDMS and SU-8 and Its Application towards a Multifunctional Microfluidic Device.Micromachines · 2016Article
- Gold Nanoparticles in Single-Cell Analysis for Surface Enhanced Raman Scattering.Molecules (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
As a complement and alternative to optical methods, wide-band electrical impedance spectroscopy (EIS) enables multi-parameter, label-free and real-time detection of cellular and subcellular features. We report on a microfluidics-based system designed to reliably capture single rod-shaped Schizosaccharomyces pombe cells by applying suction through orifices in a channel wall. The system enables subsequent culturing of immobilized cells in an upright position, while dynamic changes in cell-cycle state and morphology were continuously monitored through EIS over a broad frequency range. Besides measuring cell growth, clear impedance signals for nuclear division have been obtained. The EIS system has been characterized with respect to sensitivity and detection limits. The spatial resolution in measuring cell length was 0.25 μm, which corresponds to approximately a 5-min interval of cell growth under standard conditions. The comprehensive impedance data sets were also used to determine the occurrence of nuclear division and cytokinesis. The obtained results have been validated through concurrent confocal imaging and plausibilized through comparison with finite-element modeling data. The possibility to monitor cellular and intracellular features of single S. pombe cells during the cell cycle at high spatiotemporal resolution renders the presented microfluidics-based EIS system a suitable tool for dynamic single-cell investigations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.