ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2016
Hypothiocyanite produced by human and rat respiratory epithelial cells inactivates extracellular H1N2 influenza A virus.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Viral Comorbidities Remodel Host Transcriptome and Redox Signaling in an NADPH Oxidase Isoform-Specific Manner.Viruses · 2026Review
- Mucosal Immunity: Lessons from the Lower Respiratory and Small Intestinal Epithelia.Biomedicines · 2025Review
- The therapeutic potential of thiocyanate and hypothiocyanous acid against pulmonary infections.Free radical biology & medicine · 2024Review
- Current concepts on Pseudomonas aeruginosa interaction with human airway epithelium.PLoS pathogens · 2023Review
- Supporting the Aspecific Physiological Defenses of Upper Airways against Emerging SARS-CoV-2 Variants.Pathogens (Basel, Switzerland) · 2023Article
- Dual oxidase 1 is dispensable duringFrontiers in immunology · 2023Article
- The Hypothiocyanite and Amantadine Combination Treatment Prevents Lethal Influenza A Virus Infection in Mice.Frontiers in immunology · 2022Article
- Microbicidal Activity of Hypothiocyanite against Pneumococcus.Antibiotics (Basel, Switzerland) · 2021Article
- Dual oxidase 1 promotes antiviral innate immunity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Hypothiocyanite and Hypothiocyanite/Lactoferrin Mixture Exhibit Virucidal Activity In Vitro against SARS-CoV-2.Pathogens (Basel, Switzerland) · 2021Article
- The Role of Thiocyanate in Modulating Myeloperoxidase Activity during Disease.International journal of molecular sciences · 2020Review
- Nasal disinfection for the prevention and control of COVID-19: A scoping review on potential chemo-preventive agents.International journal of hygiene and environmental health · 2020Article
- Oxidative killing of encapsulated and nonencapsulated Streptococcus pneumoniae by lactoperoxidase-generated hypothiocyanite.PloS one · 2020Article
- Antimicrobial actions of dual oxidases and lactoperoxidase.Journal of microbiology (Seoul, Korea) · 2018Review
- Susceptibility of influenza viruses to hypothiocyanite and hypoiodite produced by lactoperoxidase in a cell-free system.PloS one · 2018Article
- Effects of orally administered lactoferrin and lactoperoxidase on symptoms of the common cold.International journal of health sciencesArticle
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
OBJECTIVE AND
designOur aim was to study whether an extracellular, oxidative antimicrobial mechanism inherent to tracheal epithelial cells is capable of inactivating influenza H1N2 virus. MATERIAL OR SUBJECTS: Epithelial cells were isolated from tracheas of male Sprague-Dawley rats. Both primary human and rat tracheobronchial epithelial cells were differentiated in air-liquid interface cultures. TREATMENT: A/swine/Illinois/02860/09 (swH1N2) influenza A virions were added to the apical side of airway cells for 1 h in the presence or absence of lactoperoxidase or thiocyanate.
methodsCharacterization of rat epithelial cells (morphology, Duox expression) occurred via western blotting, PCR, hydrogen peroxide production measurement and histology. The number of viable virions was determined by plaque assays. Statistical difference of the results was analyzed by ANOVA and Tukey's test.
resultsOur data show that rat tracheobronchial epithelial cells develop a differentiated, polarized monolayer with high transepithelial electrical resistance, mucin production and expression of dual oxidases. Influenza A virions are inactivated by human and rat epithelial cells via a dual oxidase-, lactoperoxidase- and thiocyanate-dependent mechanism.
conclusionsDifferentiated air-liquid interface cultures of rat tracheal epithelial cells provide a novel model to study airway epithelium-influenza interactions. The dual oxidase/lactoperoxidase/thiocyanate extracellular oxidative system producing hypothiocyanite is a fast and potent anti-influenza mechanism inactivating H1N2 viruses prior to infection of the epithelium.
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