ArticleScientific reports2015
Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Development of lateral flow assays to detect host proteins in cattle for improved diagnosis of bovine tuberculosis.Frontiers in veterinary science · 2023Article
- Red-Emitting Latex Nanoparticles by Stepwise Entrapment of β-Diketonate Europium Complexes.International journal of molecular sciences · 2022Article
- Photoluminescent Molecules and Materials as Diagnostic Reporters in Lateral Flow Assays.ACS applied bio materials · 2022Article
- Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy.ACS sensors · 2021Article
- Review
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains.BMC microbiology · 2020Article
- Calibration of an Upconverting Phosphor-Based Quantitative Immunochromatographic Assay for DetectingFrontiers in cellular and infection microbiology · 2020Article
- Article
- Article
- Preparation of KJournal of fluorescence · 2016Article
- Rapid detection of abrin in foods with an up-converting phosphor technology-based lateral flow assay.Scientific reports · 2016Article
- Quantitative lateral flow strip assays as User-Friendly Tools To Detect Biomarker Profiles For Leprosy.Scientific reports · 2016Article
- Rapid Identification and Characterization of Francisella by Molecular Biology and Other Techniques.The open microbiology journal · 2016Article
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay was 10(4) CFU · mL(-1) (100 CFU/test). The linear quantitative range covered five orders of magnitude, and the coefficients of variation were less than 10%. Except Shigella dysenteriae, UPT-LF showed excellent specificity to four strains that are also potential biowarfare/bioterrorism agents and 13 food-borne pathogenic strains. Samples with pH 2-13, high ion strengths (≥ 2 mol · L(-1) solution of KCl and NaCl), high viscosities (≤ 50 mg · mL(-1) PEG20000 or ≥ 20% glycerol), and high concentrations of biomacromolecules (≥ 400 mg · mL(-1) bovine serum albumin or ≥ 80 mg · mL(-1) casein) showed little influence on the assay. For practical utilization, the tolerance limits for seven powders and eight viscera were determined, and operation errors of liquid measurement demonstrated a minor influence on the strip. Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.
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