ArticleNature communications2015
An in cellulo-derived structure of PAK4 in complex with its inhibitor Inka1.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 74 citations in OpenAlex.
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- Structural basis for a p21-activated kinase 4 and nicotinamide phosphoribosyltransferase dual inhibitor.Acta crystallographica. Section D, Structural biology · 2026Article
- Programmed synthesis of mesoporous protein crystals in cellular reactors.Nature nanotechnology · 2026Article
- Mitophagy activation for jawbone radiation injury therapy via angiogenesis/osteogenesis-active nanozymes.Science China. Life sciences · 2026Article
- Review
- PAK4 phosphorylates cyclin-dependent kinase 2 to promote the GExperimental & molecular medicine · 2025Article
- Intracellular protein crystallization in living insect cells.FEBS open bio · 2025Article
- PAK4 phosphorylates and inhibits AMPKα to control glucose uptake.Nature communications · 2024Article
- High-throughput structure determination of an intrinsically disordered protein using cell-free protein crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Convolutional neural network approach for the automated identification ofJournal of applied crystallography · 2024Article
- A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.Nature communications · 2024Article
- Writing cellular history in protein chains.Nature biotechnology · 2023Article
- Time-tagged ticker tapes for intracellular recordings.Nature biotechnology · 2023Article
- The Inhibitory Mechanism of 7Molecules (Basel, Switzerland) · 2023Article
- Rho family GTPase signaling through type II p21-activated kinases.Cellular and molecular life sciences : CMLS · 2022Review
- Molecular basis for integrin adhesion receptor binding to p21-activated kinase 4 (PAK4).Communications biology · 2022Article
- Cell-free protein crystallization for nanocrystal structure determination.Scientific reports · 2022Article
- Inka2, a novel Pak4 inhibitor, regulates actin dynamics in neuronal development.PLoS genetics · 2022Article
- Large-Scale Multi-Omics Studies Provide New Insights into Blood Pressure Regulation.International journal of molecular sciences · 2022Article
- Recent advances on development of p21-activated kinase 4 inhibitors as anti-tumor agents.Frontiers in pharmacology · 2022Review
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
PAK4 is a metazoan-specific kinase acting downstream of Cdc42. Here we describe the structure of human PAK4 in complex with Inka1, a potent endogenous kinase inhibitor. Using single mammalian cells containing crystals 50 μm in length, we have determined the in cellulo crystal structure at 2.95 Å resolution, which reveals the details of how the PAK4 catalytic domain binds cellular ATP and the Inka1 inhibitor. The crystal lattice consists only of PAK4-PAK4 contacts, which form a hexagonal array with channels of 80 Å in diameter that run the length of the crystal. The crystal accommodates a variety of other proteins when fused to the kinase inhibitor. Inka1-GFP was used to monitor the process crystal formation in living cells. Similar derivatives of Inka1 will allow us to study the effects of PAK4 inhibition in cells and model organisms, to allow better validation of therapeutic agents targeting PAK4.
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